• Product nameAnti-eNOS antibody
    See all eNOS primary antibodies
  • Description
    Rabbit polyclonal to eNOS
  • SpecificityThis antibody is specific for nitric oxide synthase (NOS), derived from endothelial tissue, but does not recognize NOS derived from brain (bNOS) and macrophages (iNOS).
  • Tested applicationsSuitable for: WB, IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Cow
    Predicted to work with: Rat, Guinea pig, Human
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to internal sequence amino acids 596-609 of Cow eNOS

  • Positive control
    • Whole extract of bovine lung endothelial cells or mouse heart tissue sections.


Associated products


Our Abpromise guarantee covers the use of ab50260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 133 kDa.
IHC-Fr 1/100. Fix with acetone.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20702812


  • FunctionProduces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
  • Tissue specificityPlatelets, placenta, liver and kidney.
  • Involvement in diseaseVariation in NOS3 seem to be associated with susceptibility to coronary spasm.
  • Sequence similaritiesBelongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Post-translational
    Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
  • Cellular localizationCell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • cNOS antibody
    • Constitutive NOS antibody
    • EC NOS antibody
    • EC-NOS antibody
    • ecNOS antibody
    • Endothelial nitric oxidase synthase antibody
    • Endothelial nitric oxide synthase antibody
    • Endothelial nitric oxide synthase 3 antibody
    • Endothelial NOS antibody
    • eNOS antibody
    • Nitric oxide synthase 3 (endothelial cell) antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 endothelial cell antibody
    • Nitric oxide synthase endothelial antibody
    • Nitric oxide synthase, endothelial antibody
    • NOS 3 antibody
    • NOS III antibody
    • NOS type III antibody
    • NOS3 antibody
    • NOS3_HUMAN antibody
    • NOSIII antibody
    see all

References for Anti-eNOS antibody (ab50260)

This product has been referenced in:
  • Feng J  et al. Endothelium-dependent coronary vasodilatation requires NADPH oxidase-derived reactive oxygen species. Arterioscler Thromb Vasc Biol 30:1703-10 (2010). IHC-FoFr ; Mouse . Read more (PubMed: 20702812) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Mesentery sections)
Permeabilization Yes - Triton X-100
Specification Mesentery sections
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative Acetone

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Submitted Nov 24 2015