Overview

  • Product nameAnti-eNOS antibodySee all eNOS primary antibodies ...
  • Description
    Rabbit polyclonal to eNOS
  • SpecificityThis antibody does not detect human inducible NOS or rat brain nNOS. ab5589 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects an ~140 kDa protein representing eNOS from human endothelial cells. By Western blot ab5589 also detects eNOS in rat lung, brain, and canine cardiac microsomes. Immunohistochemical staining of eNOS in Human vascular endothelial cells with ab5589 results in prominent staining of the epithelial cells.
  • Tested applicationsICC/IF, IHC-P, IHC-Fr, IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human eNOS aa 1179-1194.
    Sequence:

    SLQERQLRGAVPWAFD

  • Positive control
    • IHC: Human endothelial cells. WB:canine cardiac microsomes and rat or human lung lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab5589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 16797727
IHC-P 1/100. Antigen retrieval is not essential but may optimise staining.
IHC-Fr 1/100.
IHC-FoFr Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 133 kDa. By Western blot ab5589 also detects eNOS in canine cardiac microsomes and rat lung.

Target

  • FunctionProduces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
  • Tissue specificityPlatelets, placenta, liver and kidney.
  • Sequence similaritiesBelongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Cellular localizationCell membrane. Membrane > caveola. Cytoplasm > cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • cNOS antibody
    • Constitutive NOS antibody
    • EC NOS antibody
    • EC-NOS antibody
    • ecNOS antibody
    • Endothelial nitric oxidase synthase antibody
    • Endothelial nitric oxide synthase antibody
    • Endothelial nitric oxide synthase 3 antibody
    • Endothelial NOS antibody
    • eNOS antibody
    • Nitric oxide synthase 3 (endothelial cell) antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 endothelial cell antibody
    • Nitric oxide synthase endothelial antibody
    • Nitric oxide synthase, endothelial antibody
    • NOS 3 antibody
    • NOS III antibody
    • NOS type III antibody
    • NOS3 antibody
    • NOS3_HUMAN antibody
    • NOSIII antibody
    see all

Anti-eNOS antibody images

  • Immunolocalization of eNOS in human lung tissue using ab5589.

References for Anti-eNOS antibody (ab5589)

This product has been referenced in:
  • Stæhr M  et al. Deletion of cyclooxygenase-2 in the mouse increases arterial blood pressure with no impairment in renal NO production in response to chronic high salt intake. Am J Physiol Regul Integr Comp Physiol 304:R899-907 (2013). Mouse . Read more (PubMed: 23535462) »
  • Zhu XY  et al. Xuezhikang, extract of red yeast rice, improved abnormal hemorheology, suppressed caveolin-1 and increased eNOS expression in atherosclerotic rats. PLoS One 8:e62731 (2013). WB ; Rat . Read more (PubMed: 23675421) »

See all 17 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step None
Sample Rat Tissue sections (Mesenteric venule)
Specification Mesenteric venule
Permeabilization Yes - Triton X-100
Fixative Paraformaldehyde
Username

Sulei Xu

Verified customer

Submitted Nov 13 2013

Application Western blot
Sample Mouse Cell lysate - whole cell (lung endothelial cells)
Loading amount 10 µg
Specification lung endothelial cells
Gel Running Conditions Reduced Denaturing (gradient gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 12 2013

Any of the following antibodies would be suitable as they are guaranteed to work in human, rat and in WB:

Anti-eNOS antibody (ab66127)
Anti-eNOS antibody (ab5589)
Anti-eNOS antibody (ab51086)
Anti-eNOS antibody [NOS-E1] (ab5001...

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Thank you very much for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge vial of ab66217 which should arrive tomorrow.

Please keep me updated about the resul...

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Thank you so much for your calls today and for your questions.

Please let me know if you would like to try any of the antibodies in ICC that haven't already been tested, and I will be happy to set up the testing discount program for you. If y...

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A while ago we spoke in regards to searching for the best antibodies to use for detection of eNOS and phospho eNOS on mouse and rat tissue, on paraffin sections (IHC-P)as well as in Western blot.
..

We have the ab66127 or the ab5589 agai...

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Thanks for your reply.

I have searched our proforma ID's and this number does not come up. I can usually find the order based on when it was placed. Do you know the date the order was placed from Sellex?

Thanks again!

Thanks for sending the email again. Not sure what is going on but the attachement still did not appear on the email. However it seems the customer is very interested in getting a replacement so I'm happy to send one.

Please let me know what a...

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Thank you for your enquiry.

I am sorry but for some reason I did not receive the positive control you mentioned, "obtained with HUVEC = CD31". If you could, please re-attach the image and forward it to me.

Thanks!

Thank you for your enquiry.

It seems as though you did not receive my reply on Monday, May 21, via CCE3784190. Please see below for the contents of the email:

=====

Thank you for submitting your completed western blot que...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"