• Product nameAnti-eNOS (phospho S116) antibody
    See all eNOS primary antibodies
  • Description
    Rabbit polyclonal to eNOS (phospho S116)
  • Specificityab78004 recognizes eNOS phosphorylated on serine 116
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Cow, Human
    Predicted to work with: Sheep, Pig
  • Immunogen

    Synthetic peptide: PRKLQGRPSPPGPP-C conjugated to KLH, corresponding to amino acids 106-118 of Human eNOS

  • Positive control
    • Fetal bovine heart endothelial cell lysate.



Our Abpromise guarantee covers the use of ab78004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).


  • FunctionProduces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
  • Tissue specificityPlatelets, placenta, liver and kidney.
  • Involvement in diseaseVariation in NOS3 seem to be associated with susceptibility to coronary spasm.
  • Sequence similaritiesBelongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Post-translational
    Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
  • Cellular localizationCell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • cNOS antibody
    • Constitutive NOS antibody
    • EC NOS antibody
    • EC-NOS antibody
    • ecNOS antibody
    • Endothelial nitric oxidase synthase antibody
    • Endothelial nitric oxide synthase antibody
    • Endothelial nitric oxide synthase 3 antibody
    • Endothelial NOS antibody
    • eNOS antibody
    • Nitric oxide synthase 3 (endothelial cell) antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 endothelial cell antibody
    • Nitric oxide synthase endothelial antibody
    • Nitric oxide synthase, endothelial antibody
    • NOS 3 antibody
    • NOS III antibody
    • NOS type III antibody
    • NOS3 antibody
    • NOS3_HUMAN antibody
    • NOSIII antibody
    see all

Anti-eNOS (phospho S116) antibody images

  • All lanes : Anti-eNOS (phospho S116) antibody (ab78004) at 1/1000 dilution

    Lane 1 : Fetal bovine heart endothelial cell lysate
    Lane 2 : Lambda phosphatase-treated fetal bovine heart endothelial cell lysate

    Goat anti-rabbit HRP conjugate

    Predicted band size : 133 kDa
    Observed band size : 133 kDa
    Additional bands at : 190 kDa. We are unsure as to the identity of these extra bands.

References for Anti-eNOS (phospho S116) antibody (ab78004)

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