Overview

  • Product nameAnti-eNOS (phospho S1177) antibody
    See all eNOS primary antibodies
  • Description
    Rabbit polyclonal to eNOS (phospho S1177)
  • SpecificityThis antibody detects endogenous levels of eNOS only when phosphorylated at serine 1176
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human eNOS (phospho S1177).

  • Positive control
    • HeLa cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab51038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
ELISA 1/20000.

Target

  • FunctionProduces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
  • Tissue specificityPlatelets, placenta, liver and kidney.
  • Involvement in diseaseVariation in NOS3 seem to be associated with susceptibility to coronary spasm.
  • Sequence similaritiesBelongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Post-translational
    modifications
    Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
  • Cellular localizationCell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • cNOS antibody
    • Constitutive NOS antibody
    • EC NOS antibody
    • EC-NOS antibody
    • ecNOS antibody
    • Endothelial nitric oxidase synthase antibody
    • Endothelial nitric oxide synthase antibody
    • Endothelial nitric oxide synthase 3 antibody
    • Endothelial NOS antibody
    • eNOS antibody
    • Nitric oxide synthase 3 (endothelial cell) antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 endothelial cell antibody
    • Nitric oxide synthase endothelial antibody
    • Nitric oxide synthase, endothelial antibody
    • NOS 3 antibody
    • NOS III antibody
    • NOS type III antibody
    • NOS3 antibody
    • NOS3_HUMAN antibody
    • NOSIII antibody
    see all

Anti-eNOS (phospho S1177) antibody images

  • All lanes : Anti-eNOS (phospho S1177) antibody (ab51038) at 1/500 dilution

    Lane 1 : HeLa cell extract treated with Insulin (0.01 U/ml for 15min), with the immunising peptide
    Lane 2 : HeLa cell extract treated with Insulin (0.01 U/ml for 15min), with the immunising peptide


    Predicted band size : 133 kDa
    Observed band size : 133 kDa

References for Anti-eNOS (phospho S1177) antibody (ab51038)

This product has been referenced in:
  • Cheang WS  et al. Endothelial nitric oxide synthase enhancer reduces oxidative stress and restores endothelial function in db/db mice. Cardiovasc Res 92:267-75 (2011). Read more (PubMed: 21875904) »

See 1 Publication for this product

Product Wall

Thank you for your patience. I managed to locate a publication that used endothelia from aorta of rats for an eNOS (phospho S1176) experiment. Therefore, this can also be used as a positive control for ab51038. Please see the Materials and Meth...

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Thank you for your enquiry. I can see from the SwissProt database entry for this protein (http://us.expasy.org/cgi-bin/niceprot.pl?P29474) that they list amino acid 1177 as being a phosphoserine. If you look at the sequence, however, they are incl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"