Overview

  • Product nameAnti-eNOS (phospho S1177) antibodySee all eNOS primary antibodies ...
  • Description
    Rabbit polyclonal to eNOS (phospho S1177)
  • SpecificityThis antibody detects endogenous levels of eNOS only when phosphorylated at serine 1176
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human eNOS (phospho S1177).

  • Positive control
    • HeLa cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab51038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/1000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
ELISA 1/20000.

Target

  • FunctionProduces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
  • Tissue specificityPlatelets, placenta, liver and kidney.
  • Sequence similaritiesBelongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Cellular localizationCell membrane. Membrane > caveola. Cytoplasm > cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
  • Target information above from: UniProt accession P29474 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • cNOS antibody
    • Constitutive NOS antibody
    • EC NOS antibody
    • EC-NOS antibody
    • ecNOS antibody
    • Endothelial nitric oxidase synthase antibody
    • Endothelial nitric oxide synthase antibody
    • Endothelial nitric oxide synthase 3 antibody
    • Endothelial NOS antibody
    • eNOS antibody
    • Nitric oxide synthase 3 (endothelial cell) antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 endothelial cell antibody
    • Nitric oxide synthase endothelial antibody
    • Nitric oxide synthase, endothelial antibody
    • NOS 3 antibody
    • NOS III antibody
    • NOS type III antibody
    • NOS3 antibody
    • NOS3_HUMAN antibody
    • NOSIII antibody
    see all

Anti-eNOS (phospho S1177) antibody images

  • All lanes : Anti-eNOS (phospho S1177) antibody (ab51038) at 1/500 dilution

    Lane 1 : HeLa cell extract treated with Insulin (0.01 U/ml for 15min), with the immunising peptide
    Lane 2 : HeLa cell extract treated with Insulin (0.01 U/ml for 15min), without the immunising peptide


    Predicted band size : 133 kDa
    Observed band size : 133 kDa

References for Anti-eNOS (phospho S1177) antibody (ab51038)

This product has been referenced in:
  • Cheang WS  et al. Endothelial nitric oxide synthase enhancer reduces oxidative stress and restores endothelial function in db/db mice. Cardiovasc Res 92:267-75 (2011). Read more (PubMed: 21875904) »

See 1 Publication for this product

Product Wall

Thank you for your patience. I managed to locate a publication that used endothelia from aorta of rats for an eNOS (phospho S1176) experiment. Therefore, this can also be used as a positive control for ab51038. Please see the Materials and Meth...

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Thank you for your enquiry. I can see from the SwissProt database entry for this protein (http://us.expasy.org/cgi-bin/niceprot.pl?P29474) that they list amino acid 1177 as being a phosphoserine. If you look at the sequence, however, they are incl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"