Overview

  • Product nameAnti-ENOX1 antibody
    See all ENOX1 primary antibodies
  • Description
    Rabbit polyclonal to ENOX1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide from within the internal sequence amino acids 468 - 517: QQLQFLQQTM QGMQQQLLTI QEELNNKKSE LEQAKEEQSH TQALLKVLQE, of Human ENOX1 (NP_060463)

  • Positive control
    • 721_B cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab83540 is purified by a peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • FunctionProbably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 24 minutes and play a role in control of the ultradian cellular biological clock.
  • Tissue specificityExpressed in lymphocyte cells, breast and breast cancer (at protein level). Found in the sera of cancer patients with a wide variety of cancers including breast, prostate, lung and ovarian cancers, leukemias, and lymphomas. Found also in the serum of healthy volunteers or patients with disorders other than cancer. Probably shed into serum by cancer cells.
  • Sequence similaritiesBelongs to the ENOX family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localizationCell membrane. Secreted > extracellular space. Extracellular and plasma membrane-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA64J21.1 antibody
    • candidate growth related and time keeping constitutive hydroquinone (NADH) oxidase antibody
    • Candidate growth related and time keeping constitutive hydroquinone [NADH] oxidase antibody
    • Candidate growth-related and time keeping constitutive hydroquinone [NADH] oxidase antibody
    • cCNOX antibody
    • Cell proliferation inducing gene 38 protein antibody
    • Cell proliferation-inducing gene 38 protein antibody
    • cNOX antibody
    • Constitutive Ecto NOX antibody
    • Constitutive Ecto-NOX antibody
    • Ecto NOX disulfide thiol exchanger 1 antibody
    • ENOX1 antibody
    • ENOX1_HUMAN antibody
    • FLJ10094 antibody
    • OTTHUMP00000018332 antibody
    • PIG38 antibody
    • Proliferation inducing protein 38 antibody
    • Protein disulfide-thiol oxidoreductase antibody
    see all

Anti-ENOX1 antibody images

  • Anti-ENOX1 antibody (ab83540) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 73 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)

References for Anti-ENOX1 antibody (ab83540)

ab83540 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"