The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Antibody detection limit dilution 1/64000.
WB: Preliminary experiments gave bands at approx 60kDa and 37kDa in Human Heart lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 66.6kDa and 70.1kDa according to NP_006366.2 and NP_872114.1. Both detected bands were successfully blocked by incubation with the immunising peptide (and BLAST results with the immunising peptide sequence did not identify any other proteins to explain the additional bands).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.
Tissue specificityFound in the sera of cancer patients with a wide variety of cancers including breast, prostate, lung and ovarian cancers, leukemias, and lymphomas. Not found in the serum of healthy volunteers or patients with disorders other than cancer. Probably shed into serum by cancer cells. Found on the cell borders of renal, kidney and ovarian carcinomas but not on the borders of surrounding non-cancerous stromal cells.
Sequence similaritiesBelongs to the ENOX family. Contains 1 RRM (RNA recognition motif) domain.