Anti-ENOX2 antibody - N-terminal (ab171420)

Overview

  • Product name
    Anti-ENOX2 antibody - N-terminal
    See all ENOX2 primary antibodies
  • Description
    Rabbit polyclonal to ENOX2 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ENOX2 aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q16206

  • Positive control
    • Jurkat cell line lysate, Human stomach tissue, Jurkat cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 70 kDa.

Target

  • Function
    May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.
  • Tissue specificity
    Found in the sera of cancer patients with a wide variety of cancers including breast, prostate, lung and ovarian cancers, leukemias, and lymphomas. Not found in the serum of healthy volunteers or patients with disorders other than cancer. Probably shed into serum by cancer cells. Found on the cell borders of renal, kidney and ovarian carcinomas but not on the borders of surrounding non-cancerous stromal cells.
  • Sequence similarities
    Belongs to the ENOX family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Cell membrane. Secreted > extracellular space. Extracellular and plasma membrane-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • APK1 antibody
    • APK1 antigen antibody
    • COVA1 antibody
    • Cytosolic ovarian carcinoma antigen 1 antibody
    • Ecto NOX disulfide thiol exchanger 2 antibody
    • ENOX 2 antibody
    • ENOX2 antibody
    • ENOX2_HUMAN antibody
    • OTTHUMP00000024021 antibody
    • OTTHUMP00000024022 antibody
    • Protein disulfide-thiol oxidoreductase antibody
    • tNOX antibody
    • Tumor associated hydroquinone oxidase antibody
    • Tumor-associated hydroquinone oxidase antibody
    see all

Images

  • Anti-ENOX2 antibody - N-terminal (ab171420) at 1/100 dilution + Jurkat cell line lysate at 35 µg

    Predicted band size: 70 kDa

  • Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling ENOX2 with ab171420 at 1/10 followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow Cytometrical analysis of Jurkat cells with ab171420 antibody at a dilution of 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

References

ab171420 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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