• Product nameAnti-ENPP3 antibody
    See all ENPP3 primary antibodies
  • Description
    Rabbit polyclonal to ENPP3
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, from the C-terminal region of Human ENPP3 (NP_005012.2).

  • Positive control
    • Human ovary and testis tissues.



Our Abpromise guarantee covers the use of ab118445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 15 - 20 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 100 kDa.


  • FunctionCleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.
  • Tissue specificityExpressed in bile ducts, prostate, uterus and colon. Exclusively expressed on basophils, mast cells and their progenitors.
  • Sequence similaritiesBelongs to the nucleotide pyrophosphatase/phosphodiesterase family.
    Contains 2 SMB (somatomedin-B) domains.
  • Post-translational
    N-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
  • Cellular localizationMembrane. Secreted. Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alkaline phosphodiesterase I antibody
    • ARHR2 antibody
    • B10 antibody
    • CD203c antibody
    • CD203c antigen antibody
    • dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody
    • dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody
    • E NPP 3 antibody
    • E-NPP 3 antibody
    • Ectonucleotide pyrophosphatase/phosphodiesterase 3 antibody
    • Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 antibody
    • ENPP3 antibody
    • ENPP3_HUMAN antibody
    • gp130RB13 6 antibody
    • gp130RB136 antibody
    • M6S1 antibody
    • NPP1 antibody
    • NPP3 antibody
    • NPPase antibody
    • NPPS antibody
    • Nucleotide pyrophosphatase antibody
    • PC-1 antibody
    • PCA1 antibody
    • PD Ibeta antibody
    • PD-Ibeta antibody
    • PDNP1 antibody
    • PDNP3 antibody
    • Phosphodiesterase I beta antibody
    • Phosphodiesterase I/nucleotide pyrophosphatase 3 antibody
    • phosphodiesterase i/nucleotide pyrophosphatase beta antibody
    see all

Anti-ENPP3 antibody images

  • All lanes : Anti-ENPP3 antibody (ab118445) at 1/1000 dilution

    Lane 1 : HEK293 cell lysate - untransfected
    Lane 2 : HEK293 cell lysate - transfected with ENNP3
    Lane 3 : HEK293 cell lysate - transfected with ENPP1

    Lysates/proteins at 10 µg per lane.

    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 100 kDa
    Observed band size : 106 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Paul Stabach

    See Abreview

  • ab118445 at 20µg/ml staining ENPP3 in Formalin-fixed, Paraffin-embedded Human ovary tissue by immunohistochemistry.
  • ab118445 at 20µg/ml staining ENPP3 in Formalin-fixed, Paraffin-embedded Human testis tissue by immunohistochemistry.

References for Anti-ENPP3 antibody (ab118445)

ab118445 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - other (HEK293)
Specification HEK293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Dr. Paul Stabach

Verified customer

Submitted Sep 12 2014