Anti-ENPP3 antibody [NP4D6] (Phycoerythrin) (ab90751)

Overview

  • Product nameAnti-ENPP3 antibody [NP4D6] (Phycoerythrin)
    See all ENPP3 primary antibodies
  • Description
    Mouse monoclonal [NP4D6] to ENPP3 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    HEK-293 cells transfected with Human ENPP3

  • General notes

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.

Properties

Applications

Our Abpromise guarantee covers the use of ab90751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
ICC/IF
  • Application notesFlow Cyt: Use 20µl for 106 cells.
    ICC/IF: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.
    • Tissue specificityExpressed in bile ducts, prostate, uterus and colon. Exclusively expressed on basophils, mast cells and their progenitors.
    • Sequence similaritiesBelongs to the nucleotide pyrophosphatase/phosphodiesterase family.
      Contains 2 SMB (somatomedin-B) domains.
    • Post-translational
      modifications
      N-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal.
      It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    • Cellular localizationMembrane. Secreted. Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alkaline phosphodiesterase I antibody
      • ARHR2 antibody
      • B10 antibody
      • CD203c antibody
      • CD203c antigen antibody
      • dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody
      • dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody
      • E NPP 3 antibody
      • E-NPP 3 antibody
      • Ectonucleotide pyrophosphatase/phosphodiesterase 3 antibody
      • Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 antibody
      • ENPP3 antibody
      • ENPP3_HUMAN antibody
      • gp130RB13 6 antibody
      • gp130RB136 antibody
      • M6S1 antibody
      • NPP1 antibody
      • NPP3 antibody
      • NPPase antibody
      • NPPS antibody
      • Nucleotide pyrophosphatase antibody
      • PC-1 antibody
      • PCA1 antibody
      • PD Ibeta antibody
      • PD-Ibeta antibody
      • PDNP1 antibody
      • PDNP3 antibody
      • Phosphodiesterase I beta antibody
      • Phosphodiesterase I/nucleotide pyrophosphatase 3 antibody
      • phosphodiesterase i/nucleotide pyrophosphatase beta antibody
      see all

    Anti-ENPP3 antibody [NP4D6] (Phycoerythrin) images

    • ab90751 staining ENPP3 on the surface of human basophils isolated from allergen-stimulated whole blood by flow cytometery.

    • Surface staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, ab18235, green) and PE-conjugated anti-ENPP3 (clone NP4D6, ab90751, red); merged signal yellow. DNA visualized by DAPI (blue).

    References for Anti-ENPP3 antibody [NP4D6] (Phycoerythrin) (ab90751)

    ab90751 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Fixation no fixation
    Permeabilization No
    Sample Human Cell (human basophile granulocytes)
    Specification human basophile granulocytes
    Gating Strategy leucocyte gate
    Preparation Cell harvesting/tissue preparation method: peripheral blood cells, separated by Ficoll density separation, followed by magnetic isolation
    Sample buffer: PBS with human IgG (Fc-block)
    Username

    Abcam user community

    Verified customer

    Submitted Aug 15 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Sample Human Cell (peripheral blood cells)
    Specification peripheral blood cells
    Permeabilization No
    Fixative Methanol
    Username

    Abcam user community

    Verified customer

    Submitted Aug 15 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (PBMC)
    Specification PBMC
    Preparation Cell harvesting/tissue preparation method: Ficoll-hypaque gradrient
    Sample buffer: PBS
    Permeabilization No
    Gating Strategy lymphocytes
    Username

    Abcam user community

    Verified customer

    Submitted Feb 19 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"