Recombinant Anti-NPP-7 antibody [EPR12329] (ab174281)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12329] to NPP-7
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NPP-7 antibody [EPR12329]
See all NPP-7 primary antibodies -
Description
Rabbit monoclonal [EPR12329] to NPP-7 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human NPP-7 aa 350-450 (Cysteine residue). The exact sequence is proprietary.
Database link: Q6UWV6 -
Positive control
- Caco 2, HT-29 and Human fetal liver lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12329 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 51 kDa.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 51 kDa. |
Target
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Function
Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. -
Tissue specificity
Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon. -
Sequence similarities
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. -
Post-translational
modificationsN-glycosylated; required for activity and transport to the plasma membrane. -
Cellular localization
Membrane. Localized at the surface of the microvillar membrane in small intestine enterocytes, and in endosome-like structures situated beneath the microvillar membrane, and in Golgi complex. - Information by UniProt
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Database links
- Entrez Gene: 339221 Human
- Entrez Gene: 238011 Mouse
- SwissProt: Q6UWV6 Human
- Unigene: 114084 Human
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Alternative names
- ALK SMase antibody
- Alk-SMase antibody
- Alkaline sphingomyelin phosphodiesterase antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab174281 has not yet been referenced specifically in any publications.