Anti-Enrofloxacin/Ciprofloxacin antibody [72FIG1F7_1] (ab21035)

Overview

  • Product nameAnti-Enrofloxacin/Ciprofloxacin antibody [72FIG1F7_1]
  • Description
    Mouse monoclonal [72FIG1F7_1] to Enrofloxacin/Ciprofloxacin
  • Tested applicationsSuitable for: ELISAmore details
  • Immunogen

    Chemical/ Small Molecule: Enrofloxacin conjugated to KLH (keyhole limpet hemocyanin) via carbodiimide.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityPurified IgM
  • ClonalityMonoclonal
  • Clone number72FIG1F7_1
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Target

  • RelevanceEnrofloxacin is a fluoroquinolone that inhibits the activity of bacterial DNA gyrase. It was the first fluoroquinolone antimicrobial to be used in veterinary infections by E. coli, Salmonella, Pasteurella, Mycoplasma and Hemophilus species. Ciprofloxacin, a fluoroquinolone, is a concentration dependent bactericidal agent. It is structurally related to enrofloxacin, and has a similar spectrum of activity. Both of these antimicrobials have shown activity against some gram positive aerobes and a wide range of gram negative bacilli and cocci, which include Klebsiella, Pseudomonas, Salmonella, and other organisms such as Mycoplasma, Staphylococci and Chlamydia. Due to the fluoroquinolone’s variable activity against most Streptococci, as well as their weak activity against many anaerobic bacteria, they are generally not recommended for use in treating infections where these types of microbes are present. Like enrofloxacin, ciprofloxacin is believed to act by inhibiting bacterial DNA gyrase which prevents DNA supercoiling and DNA synthesis.
  • Alternative names
    • Ciprofloxacin antibody
    • Enrofloxacin antibody

References for Anti-Enrofloxacin/Ciprofloxacin antibody [72FIG1F7_1] (ab21035)

ab21035 has not yet been referenced specifically in any publications.

Product Wall

Application ELISA
Sample Mouse Purified protein (antibody)
Specification antibody
Type Sandwich (Detection)
Username

Mr. young hyun kim

Verified customer

Submitted May 02 2014

Thank you for contacting us.

The following information, I have received from laboratory;

when performing an ELISA with catalogue ab21035 enrofloxacin or ciprofloxacin conjugated to BSA is used – small haptens like these drugs h...

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Thank you for your enquiry.

As far as we know ab21035 has only been tested in ELISA, where it has successfully detected the immunogen bound to a microtiter plate well. The immunogen is "Enrofloxacin conjugated to KLH (keyhole limpet hemo...

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Thank you for contacting us.

I am sorry but I do not fully understand what the customer is hoping to achieve and have thereforefound it difficult to answer your question. How are they testing the antibodies with nanoparticles?

It is...

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Thank you for your inquiry. Indeed, antibodies as most (unmodified) proteins have a carboxy group (-COOH) at their C-terminus, as you indicated. They also have an amine group (-NH2) at their N-terminus. Furthermore, antibodies contain amino acids w...

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Thank you for your enquiry. I am sorry the customer has some concerns regarding the results of their experiment. We would not recommend to store the antigen-antibody samples in distilled water. The sample and the antibody binding are likely to be m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"