Overview

  • Product name
  • Description
    Rabbit polyclonal to Ensconsin
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 150 and 200 of human Ensconsin (NP_003971.1).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab80416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Microtubule-stabilizing protein that may play an important role during reorganization of microtubules during polarization and differentiation of epithelial cells. Associates with microtubules in a dynamic manner. May play a role in the formation of intercellular contacts. Colocalization with TRPV4 results in the redistribution of TRPV4 toward the membrane and may link cytoskeletal microfilaments.
    • Tissue specificity
      Expressed in the skin and cells of epithelial origin. Predominantly expressed in the suprabasal layers of the normal epidermis and relatively abundant in squamous cell carcinomas but barely detectable in basal cell carcinomas.
    • Sequence similarities
      Belongs to the MAP7 family.
    • Post-translational
      modifications
      The association with microtubules is regulated by phosphorylation during the cell cycle. During interphase only phosphorylated on serine. Phosphorylated on threonine in mitosis.
    • Cellular localization
      Cytoplasm > perinuclear region. Basolateral cell membrane. Cytoplasm > cytoskeleton. Colocalized on microtubules. An intracellular redistribution is triggered during induction of keratinocyte terminal differentiation from microtubules with a perinuclear localization to cortical microtubules organized in spike-like bundles facing intercellular contacts.
    • Information by UniProt
    • Database links
    • Alternative names
      • dJ325F22.2 (microtubule-associated protein 7 (EMAP115, E-MAP- 115)) antibody
      • E MAP 115 antibody
      • E-MAP-115 antibody
      • EMAP115 antibody
      • Ensconsin antibody
      • Epithelial microtubule associated protein of 115 kDa antibody
      • Epithelial microtubule-associated protein of 115 kDa antibody
      • MAP 7 antibody
      • MAP-7 antibody
      • Map7 antibody
      • MAP7_HUMAN antibody
      • Microtubule associated protein 7 antibody
      • Microtubule-associated protein 7 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Ensconsin with ab80416 at 1/1000 (1µg/ml). Detection: DAB.

    References

    ab80416 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab80416.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up