Overview

  • Product name
    Anti-ENSP00000299415 antibody
  • Description
    Rabbit polyclonal to ENSP00000299415
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: EDAFCEMNGA LVQQEEQAAR VRQRLREEED RGIVRNKVLT FLLPREKQLR EHCKRLEDLL LDRGRDALRA TKKSQAD, corresponding to amino acids 77-153 of Human ENSP00000299415

  • Positive control
    • Human liver tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

  • Database links
  • Alternative names
    • 1700106J16Rik antibody
    • Coiled-coil domain-containing protein ENSP00000299415 antibody
    • ENSP00000299415 antibody
    • RIKEN cDNA 1700106J16 gene antibody
    • RP24-402O2.1 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in golgi. Recommended concentration of ab121753 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121753 at 1/300 dilution staining ENSP00000299415 in Paraffin-emebedded Human liver tissue by Immunohistochemistry.

References

ab121753 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121753.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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