The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human ENT1 peptide (ab48606).
Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
Belongs to the SLC29A transporter family.
Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.
solute carrier family 29 (equilibrative nucleoside transporter), member 1 antibody
solute carrier family 29 (nucleoside transporters), member 1 antibody
Solute carrier family 29 member 1 antibody
Western blot - ENT1 antibody (ab48607)
All lanes : Anti-ENT1 antibody (ab48607) at 2 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431) Lane 2 : Human brain tissue lysate - total protein (ab29466) Lane 3 : Human heart tissue lysate - total protein (ab29431) with Human ENT1 peptide (ab48606) at 2 µg/ml Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human ENT1 peptide (ab48606) at 2 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Osgood CL et al. (18)F-FLT Positron Emission Tomography (PET) is a Pharmacodynamic Marker for EWS-FLI1 Activity and Ewing Sarcoma. Sci Rep6:33926 (2016).
Read more (PubMed: 27671553) »
Bicket A et al. Novel regulation of equlibrative nucleoside transporter 1 (ENT1) by receptor-stimulated Ca2+-dependent calmodulin binding. Am J Physiol Cell Physiol310:C808-20 (2016).
Read more (PubMed: 27009875) »