Overview

  • Product name
  • Description
    Rabbit polyclonal to ENT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human ENT1.

    (Peptide available as ab48606.)

  • Positive control
    • This antibody gave a positive signal in Human Heart Tissue Lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab48607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human ENT1 peptide (ab48606).

Target

  • Function
    Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
  • Tissue specificity
    Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
  • Sequence similarities
    Belongs to the SLC29A transporter family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Equilibrative NBMPR-sensitive nucleoside transporter antibody
    • equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter antibody
    • Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter antibody
    • Equilibrative nucleoside transporter 1 antibody
    • es-type antibody
    • MGC1465 antibody
    • MGC3778 antibody
    • Nucleoside transporter antibody
    • Nucleoside transporter, es-type antibody
    • OTTHUMP00000016506 antibody
    • OTTHUMP00000016507 antibody
    • OTTHUMP00000016508 antibody
    • OTTHUMP00000016509 antibody
    • OTTHUMP00000016510 antibody
    • OTTHUMP00000016511 antibody
    • OTTHUMP00000016512 antibody
    • S29A1_HUMAN antibody
    • Slc29a1 antibody
    • solute carrier family 29 (equilibrative nucleoside transporter), member 1 antibody
    • solute carrier family 29 (nucleoside transporters), member 1 antibody
    • Solute carrier family 29 member 1 antibody
    see all

References

This product has been referenced in:
  • Osgood CL  et al. (18)F-FLT Positron Emission Tomography (PET) is a Pharmacodynamic Marker for EWS-FLI1 Activity and Ewing Sarcoma. Sci Rep 6:33926 (2016). Read more (PubMed: 27671553) »
  • Bicket A  et al. Novel regulation of equlibrative nucleoside transporter 1 (ENT1) by receptor-stimulated Ca2+-dependent calmodulin binding. Am J Physiol Cell Physiol 310:C808-20 (2016). Read more (PubMed: 27009875) »

See all 7 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 #U251 #MCF-7)
Loading amount
30 µg
Specification
HEK293 #U251 #MCF-7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Sep 17 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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