Overview

  • Product name
    Anti-ENT1 antibody - N-terminal
    See all ENT1 primary antibodies
  • Description
    Goat polyclonal to ENT1 - N-terminal
  • Host species
    Goat
  • Specificity
    Reported variants represent identical protein: NP_001071642.1, NP_001071645.1, NP_001071644.1, NP_004946.1, NP_001071643.1.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human ENT1 aa 3-15 (N terminal) (Cysteine residue). (NP_001071643.1).
    Sequence:

    TSHQPQDRYKAVW


    Database link: Q99808

  • Positive control
    • Human breast, spleen and placenta lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab190877 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).

Target

  • Function
    Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
  • Tissue specificity
    Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
  • Sequence similarities
    Belongs to the SLC29A transporter family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Equilibrative NBMPR-sensitive nucleoside transporter antibody
    • equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter antibody
    • Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter antibody
    • Equilibrative nucleoside transporter 1 antibody
    • es-type antibody
    • MGC1465 antibody
    • MGC3778 antibody
    • Nucleoside transporter antibody
    • Nucleoside transporter, es-type antibody
    • OTTHUMP00000016506 antibody
    • OTTHUMP00000016507 antibody
    • OTTHUMP00000016508 antibody
    • OTTHUMP00000016509 antibody
    • OTTHUMP00000016510 antibody
    • OTTHUMP00000016511 antibody
    • OTTHUMP00000016512 antibody
    • S29A1_HUMAN antibody
    • Slc29a1 antibody
    • solute carrier family 29 (equilibrative nucleoside transporter), member 1 antibody
    • solute carrier family 29 (nucleoside transporters), member 1 antibody
    • Solute carrier family 29 member 1 antibody
    see all

Images

  • Anti-ENT1 antibody - N-terminal (ab190877) at 1 µg/ml + Human placenta lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 50 kDa
    Observed band size: 55 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.

References

ab190877 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab190877.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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