• Product nameAnti-ENT2 antibody
    See all ENT2 primary antibodies
  • Description
    Rabbit polyclonal to ENT2
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human ENT2.

    (Peptide available as ab48695.)

  • Positive control
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: Raji This antibody also gave a positive signal in the following Human Tissue Lysates: Brain, Heart This antibody also gave a positive signal in the following Mouse Tissue Lysates: Heart, Kidney It also gave a positive result in SKNSH cell line.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab48595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 20799341
ICC/IF Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).


  • FunctionMediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. Very less sensitive than SLC29A1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyridamole, dilazep and draflazine.
  • Tissue specificityExpressed in skeletal muscle, liver, lung, placenta, brain, heart, kidney and ovarian tissues.
  • Sequence similaritiesBelongs to the SLC29A/ENT transporter (TC 2.A.57) family.
  • Cellular localizationBasolateral cell membrane. Nucleus membrane. Localized at the basolateral cell membrane in polarized MDCK cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 36 kDa antibody
    • 36 kDa nucleolar protein HNP36 antibody
    • Delayed-early response protein 12 antibody
    • DER12 antibody
    • ei-type antibody
    • Equilibrative NBMPR-insensitive nucleoside transporter antibody
    • Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter antibody
    • Equilibrative nucleoside transporter 2 antibody
    • HNP36 antibody
    • Hydrophobic nucleolar protein antibody
    • Hydrophobic nucleolar protein, 36 kDa antibody
    • Nucleoside transporter antibody
    • S29A2_HUMAN antibody
    • SLC29A2 antibody
    • solute carrier family 29 (nucleoside transporters), member 29 antibody
    • Solute carrier family 29 member 2 antibody
    see all

Anti-ENT2 antibody images

  • All lanes : Anti-ENT2 antibody (ab48595) at 1 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Kidney (Mouse) Tissue Lysate
    Lane 3 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)
    Lane 5 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,11 kDa,250 kDa,45 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab48595 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • ICC/IF image of ab48595 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48595, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-ENT2 antibody (ab48595)

This product has been referenced in:
  • Grenz A  et al. Equilibrative nucleoside transporter 1 (ENT1) regulates postischemic blood flow during acute kidney injury in mice. J Clin Invest 122:693-710 (2012). IHC-Fr ; Mouse . Read more (PubMed: 22269324) »
  • Chen CF  et al. Overlapping high-resolution copy number alterations in cancer genomes identified putative cancer genes in hepatocellular carcinoma. Hepatology 52:1690-701 (2010). IHC-Fr ; Human . Read more (PubMed: 20799341) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (multiple tissues)
Loading amount 40 µg
Specification multiple tissues
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Kris Frese

Verified customer

Submitted Jan 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Cervix)
Loading amount 35 µg
Specification Cervix
Gel Running Conditions Reduced Denaturing (4-20% gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Lucy Hodge

Verified customer

Submitted Feb 25 2010