This antibody gave a positive signal in the following Human Whole Cell Lysates:
This antibody also gave a positive signal in the following Human Tissue Lysates:
This antibody also gave a positive signal in the following Mouse Tissue Lysates:
It also gave a positive result in SKNSH cell line.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 20799341
Use a concentration of 5 - 10 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
FunctionMediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. Very less sensitive than SLC29A1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyridamole, dilazep and draflazine.
Tissue specificityExpressed in skeletal muscle, liver, lung, placenta, brain, heart, kidney and ovarian tissues.
Sequence similaritiesBelongs to the SLC29A/ENT transporter (TC 2.A.57) family.
Cellular localizationBasolateral cell membrane. Nucleus membrane. Localized at the basolateral cell membrane in polarized MDCK cells.
solute carrier family 29 (nucleoside transporters), member 29 antibody
Solute carrier family 29 member 2 antibody
Anti-ENT2 antibody images
Western blot - Anti-ENT2 antibody (ab48595)
All lanes : Anti-ENT2 antibody (ab48595) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate Lane 2 : Kidney (Mouse) Tissue Lysate Lane 3 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 4 : Human brain tissue lysate - total protein (ab29466) Lane 5 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution Developed using the ECL technique
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab48595 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ICC/IF image of ab48595 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48595, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-ENT2 antibody (ab48595)
This product has been referenced in:
Zheng X et al. Epithelial-to-mesenchymal transition is dispensable for metastasis but induces chemoresistance in pancreatic cancer. Nature527:525-30 (2015).
Read more (PubMed: 26560028) »
Grenz A et al. Equilibrative nucleoside transporter 1 (ENT1) regulates postischemic blood flow during acute kidney injury in mice. J Clin Invest122:693-710 (2012).
Read more (PubMed: 22269324) »