Customer reviews and FAQs

Overview

  • Product name
    Anti-Entactin antibody
    See all Entactin primary antibodies
  • Description
    Rabbit polyclonal to Entactin
  • Tested applications
    Suitable for: WB, IHC-Fr, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein (Mouse).

Properties

Applications

Our Abpromise guarantee covers the use of ab14511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 137 kDa.
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF 1/200. PubMed: 23409002

Target

  • Function
    Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
  • Sequence similarities
    Contains 6 EGF-like domains.
    Contains 4 LDL-receptor class B repeats.
    Contains 1 NIDO domain.
    Contains 1 nidogen G2 beta-barrel domain.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • Enactin antibody
      • Ent antibody
      • Entactin 1 antibody
      • Entactin antibody
      • NID 1 antibody
      • NID antibody
      • NID-1 antibody
      • Nid1 antibody
      • NID1_HUMAN antibody
      • Nidogen (enactin) antibody
      • Nidogen 1 antibody
      • Nidogen antibody
      • Nidogen-1 antibody
      see all

    Anti-Entactin antibody images

    • Immunohistochemistry (Frozen sections) - Entactin antibody (ab14511)
      Immunohistochemistry (Frozen sections) - Entactin antibody (ab14511)Image courtesy of an anonymous Abreview.
      ab14511 staining Entactin in rat heart endocardium by Immunohistochemistry (Frozen sections)
      Tissue was fixed in acetone and permeabilized using Tween 20. Samples were then blocked with 5% serum for 1 hour at room temperature and then incubated with ab14511 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an undiluted chicken anti-rabbit IgG conjugated to Alexa Fluor 647 (blue).

      See Abreview

    References for Anti-Entactin antibody (ab14511)

    This product has been referenced in:
    • Herrera C  et al. Effects of PI and PIII Snake Venom Haemorrhagic Metalloproteinases on the Microvasculature: A Confocal Microscopy Study on the Mouse Cremaster Muscle. PLoS One 11:e0168643 (2016). IHC ; Mouse . Read more (PubMed: 27992592) »
    • Longmate WM  et al. Reduced Fibulin-2 Contributes to Loss of Basement Membrane Integrity and Skin Blistering in Mice Lacking Integrin a3ß1 in the Epidermis. J Invest Dermatol N/A:N/A (2014). Mouse . Read more (PubMed: 24390135) »

    See all 5 Publications for this product

    Publishing research using ab14511? Please let us know so that we can cite the reference in this datasheet.

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    3 Abreviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Entactin antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (retinal tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Heat mediated antigen retrieval solution pH: 6 - 250 ml abcam ref ab973)
    Permeabilization
    Yes - 0.1% Tween 20 during 5 min
    Specification
    retinal tissue
    Blocking step
    Protein Block serum-free DAKO x 0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Metanol 95% C/ Acético Glacial 5%
    Read More
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    Dr. Patricia Bogdanov

    Verified customer

    Submitted Jul 18 2016

    Dear technical officer, Here I am writing to enquire about the concentration of the following 2 antibodies (rabbit anti-nidogen1 (Ab14511) and rabbit anti-nidogen2 (Ab14513)as I will need the information to determine the concentration for the isotype c...

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    Thank you for your enquiry. I can confirm that both ab14511 and ab14513 are sold as whole antiserum. As antibody concentration is usually determined by a protein assay, I am sorry it is not possible to provide a concentration for antibody in antiser...

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    Immunohistochemistry (Frozen sections) abreview for Anti-Entactin antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Frozen heart endocardium, 5 micron thick)
    Specification
    Frozen heart endocardium, 5 micron thick
    Fixative
    Acetone
    Permeabilization
    Yes - Tween-20
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Jul 15 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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