Overview

  • Product nameAnti-Entactin antibody
    See all Entactin primary antibodies
  • Description
    Mouse monoclonal to Entactin
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Macaque Monkey
  • Immunogen

    GST-tagged recombinant fragment: EGLQYPFAVT SYGKNLYFTD WKMNSVVALD LAISKETDAF QPHKQTRLYG ITTALSQCPQ GHNYCSVNNG GCTHLCLATP GSRTCRCPDN TLGVDCIEQK, corresponding to C terminal amino acids 1148-1247 of Human Entactin (NP_002499

  • Positive control
    • Entactin transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 136 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionSulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
  • Sequence similaritiesContains 6 EGF-like domains.
    Contains 4 LDL-receptor class B repeats.
    Contains 1 NIDO domain.
    Contains 1 nidogen G2 beta-barrel domain.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Enactin antibody
    • Ent antibody
    • Entactin 1 antibody
    • Entactin antibody
    • NID 1 antibody
    • NID antibody
    • NID-1 antibody
    • Nid1 antibody
    • NID1_HUMAN antibody
    • Nidogen (enactin) antibody
    • Nidogen 1 antibody
    • Nidogen antibody
    • Nidogen-1 antibody
    see all

Anti-Entactin antibody images

  • Anti-Entactin antibody (ab77179) at 5 µg/ml + immunogen (GST-tagged 100 aa recombinant fragment) at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

    Predicted band size : 136 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Entactin antibody (ab77179) at 5 µg/ml

    Lane 1 : Entactin transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

    Predicted band size : 136 kDa
    Observed band size : 136 kDa

References for Anti-Entactin antibody (ab77179)

ab77179 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"