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Products:Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
Overview
Product name
Anti-Entactin antibody [JF2]
See all Entactin products (8) ...
Description
Rat monoclonal [JF2] to Entactin
Specificity
Recognises native and denatured Nidogen 1.
Tested applications
WB, ELISA, Blockingmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant fragment corresponding to the G3 region of mouse Nidogen 1.
Epitope
Located in the G3 region of Nidogen 1.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 2M Tris, 0.5M Acetic acid
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
JF2
Myeloma
YB2/0
Isotype
IgG2a
Research areas
Cell Biology >> Cell Cycle >> Cell differentiation
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
Applications
Show applications key
Our Abpromise guarantee covers the use of ab26847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ELISA
Blocking
Application notes
BL: Use at an assay dependent dilution. ab26847 blocks binding of nidogen to laminin.
ELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 136 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Sequence similarities
Contains 6 EGF-like domains.
Contains 4 LDL-receptor class B repeats.
Contains 1 NIDO domain.
Contains 1 nidogen G2 beta-barrel domain.
Contains 1 thyroglobulin type-1 domain.
Post-translational
modifications
N- and O-glycosylated.
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane.
Target information above from: UniProt accessionP14543
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Ent antibody
- Entactin 1 antibody
- Entactin antibody
see all
Database links
- Entrez Gene: 4811 Human
- Entrez Gene: 18073 Mouse
- Omim: 131390 Human
- SwissProt: P14543 Human
- SwissProt: P10493 Mouse
- Unigene: 356624 Human
- Unigene: 4691 Mouse
References for Anti-Entactin antibody [JF2] (ab26847)
ab26847 has not yet been referenced specifically in any publications.
Publishing research using ab26847? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"