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Abcam's Enterokinase Activity Assay Kit (Fluorometric) utilizes a peptide substrate containing the Enterokinase recognition sequence along with a fluorescent label ‘AFC’. Enterokinase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. This assay kit is simple and rapid and can detect Enterokinase activity as low as 1 mU.
Enterokinase (Enteropeptidase, EC 18.104.22.168) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of Enterokinase has been utilized in cleaving a variety of native or fusion protein tags containing the above recognition motif.
|AFC Standard||Yellow||1 x 100µl|
|Enteropeptidase Assay Buffer||WM||1 x 20ml|
|Enteropeptidase Substrate||Red||1 x 0.2ml|
|Human Enteropeptidase||Green||1 x 17µl|
Our Abpromise guarantee covers the use of ab170964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab170964 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"