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Abcam's Enterokinase Inhibitor Screening Kit (Fluorometric) utilizes a peptide substrate containing the Enteropeptidase recognition sequence along with a fluorescent label ‘AFC’. Enteropeptidase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. In the presence of potent Enteropeptidase inhibitor, the hydrolyzation of the substrate will be impeded. The kit provides a rapid, simple and reliable test for screening potential inhibitors of Enteropeptidase.
Enteropeptidase (Enterokinase, EC 188.8.131.52) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion proteins containing the above recognition.
|Enteropeptidase Assay Buffer||WM||1 x 20ml|
|Enteropeptidase Substrate||Red||1 x 0.2ml|
|Enteropeptidase-specific Inhibitor [Aprotinin]||Blue||1 x 0.1ml|
|Human Enteropeptidase||Green||1 x 0.17ml|
Our Abpromise guarantee covers the use of ab170965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab170965 has not yet been referenced specifically in any publications.