1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use citrate buffer pH 6.
FunctionIn the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent. The order of activity with different substrates is ATP > GTP > CTP = ITP > UTP >> ADP = UDP.
Tissue specificityBrain, placenta, skeletal muscle, kidney, pancreas, heart, ovary, testis, colon, small intestine, prostate and pancreas. No expression in adult thymus, spleen, lung, liver and peripheral blood leukocytes.
Sequence similaritiesBelongs to the GDA1/CD39 NTPase family.