Overview

  • Product nameAnti-ENTPD5 antibody [EPR3784]
    See all ENTPD5 primary antibodies
  • Description
    Rabbit monoclonal [EPR3784] to ENTPD5
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • Positive control
    • Fetal liver cell lysate; Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa).Can be blocked with ENTPD5 peptide (ab190069).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionUridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. Also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.
    • Tissue specificityExpressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues.
    • PathwayProtein modification; protein glycosylation.
    • Sequence similaritiesBelongs to the GDA1/CD39 NTPase family.
    • Post-translational
      modifications
      N-glycosylated; high-mannose type.
    • Cellular localizationEndoplasmic reticulum lumen.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD39 like 4 antibody
      • AI196558 antibody
      • AI987697 antibody
      • CD39 antigen like 4 antibody
      • CD39 antigen-like 4 antibody
      • CD39 like 4 antibody
      • CD39L4 antibody
      • Ectonucleoside triphosphate diphosphohydrolase 5 antibody
      • ENTP5_HUMAN antibody
      • Entpd5 antibody
      • ER UDPase antibody
      • ER-UDPase antibody
      • GDPase ENTPD5 antibody
      • Guanosine-diphosphatase ENTPD5 antibody
      • MGC163357 antibody
      • MGC163359 antibody
      • mNTPase antibody
      • NTPDase 5 antibody
      • Nucleoside diphosphatase antibody
      • PCPH antibody
      • Pcph proto oncogene protein antibody
      • Proto oncogene CPH antibody
      • UDPase ENTPD5 antibody
      • Uridine-diphosphatase ENTPD5 antibody
      see all

    Anti-ENTPD5 antibody [EPR3784] images

    • Anti-ENTPD5 antibody [EPR3784] (ab108603) at 1/1000 dilution + fetal liver cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 48 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    • ab108603, at 1/100 dilution, staining ENTPD5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    References for Anti-ENTPD5 antibody [EPR3784] (ab108603)

    This product has been referenced in:
    • Walsh J  et al. Identification and quantification of the basal and inducible Nrf2-dependent proteomes in mouse liver: biochemical, pharmacological and toxicological implications. J Proteomics 108:171-87 (2014). WB ; Mouse . Read more (PubMed: 24859727) »
    • Curry NL  et al. Pten-null tumors cohabiting the same lung display differential AKT activation and sensitivity to dietary restriction. Cancer Discov 3:908-21 (2013). WB, IHC-P ; Mouse . Read more (PubMed: 23719831) »

    See all 2 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample Mouse Cell (Spermatozoa)
    Specification Spermatozoa
    Permeabilization Yes - Triton X 0.25%
    Fixative Paraformaldehyde
    Username

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    Verified customer

    Submitted May 30 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"