The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/1000 - 1/10000. Detects a band of approximately 210 kDa (predicted molecular weight: 232 kDa).
Envoplakin is a component of the cornified envelope (epidermal barrier) and a member of the plakin family of cytoskeletal linker proteins. It is exclusively expressed in stratified squamous epithelia and is induced during differentiation of epidermal keratinocytes.
Cell junction; desmosome. Note=Colocalized with DSP at desmosomes and along intermediate filaments.
Flow Cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling Envoplakin with ab185217 at 1/110 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.