Overview

  • Product nameAnti-ENY2 antibody
    See all ENY2 primary antibodies
  • Description
    Rabbit polyclonal to ENY2
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: AINQKLMETG ERERLKELLR AKLIECGWKD QLKAHCKEVI KEKGLEHVTV DDLVAEITPK GRALVPDSVK KELLQRIRT, corresponding to amino acids 15-93 of Human ENY2.

  • Positive control
    • Human rectum tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab121836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionComponent of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates to a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. May also participate in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.
  • Sequence similaritiesBelongs to the ENY2 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1810057B09Rik antibody
    • 6720481I12 antibody
    • DC6 antibody
    • e(y)2 antibody
    • Enhancer of yellow 2 transcription factor homolog antibody
    • eny2 antibody
    • ENY2_HUMAN antibody
    • Ey2 antibody
    see all

Anti-ENY2 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab121836 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121836 at 1/40 dilution staining ENY2 in Paraffin-embedded Human Rectum tissue by Immunohistochemistry. Note: strong positivity in glandular cells.

References for Anti-ENY2 antibody (ab121836)

ab121836 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"