Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 13 kDa. The detection limit for recombinant mouse Eotaxin 2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Use a concentration of 7 - 8 µg/ml. To yield one-half maximal inhibition [ND50] of the biological activity of mouse Eotaxin 2 (100 ng/ml).
Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.
Activated monocytes and activated T lymphocytes.
Belongs to the intercrine beta (chemokine CC) family.