Anti-Eotaxin 3 antibody (Biotin) (ab83505)

Overview

  • Product nameAnti-Eotaxin 3 antibody (Biotin)
    See all Eotaxin 3 primary antibodies
  • Description
    Goat polyclonal to Eotaxin 3 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Highly pure (>98%), recombinant full length Eotaxin 3 protein (Mouse)

Properties

  • FormLyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab83505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.25 - 1 µg/ml.
Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to Eotaxin 3 (ab109612).
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa.

Target

  • FunctionChemotactic for eosinophils and basophils. Binds to CCR3.
  • Tissue specificityUbiquitously expressed at low levels in various tissues including heart and ovary.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-C motif chemokine 26 antibody
    • CC chemokine IMAC antibody
    • CCL 26 antibody
    • CCL26 antibody
    • CCL26_HUMAN antibody
    • Chemokine (C C motif) ligand 26 antibody
    • chemokine N1 antibody
    • Eotaxin-3 antibody
    • IMAC antibody
    • Macrophage inflammatory protein 4-alpha antibody
    • MIP 4a antibody
    • MIP 4alpha antibody
    • MIP-4-alpha antibody
    • MIP4a antibody
    • MIP4alpha antibody
    • SCYA 26 antibody
    • SCYA26 antibody
    • Small inducible cytokine A26 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 26 antibody
    • Small inducible cytokine subfamily A member 26 antibody
    • Small-inducible cytokine A26 antibody
    • Thymic stroma chemokine 1 antibody
    • Thymic stroma chemokine-1 antibody
    • TSC 1 antibody
    • TSC-1 antibody
    • TSC1 antibody
    see all

Anti-Eotaxin 3 antibody (Biotin) images

  • ab83505 detecting Eotaxin 3 in Eosinophil granulocytes from patients by ELISA. Samples were blocked with 1% BSA for 1 hour at 25°C and incubated with the primary antibody (1/2000 in 1% BSA in PBS) for 2 hours at 25°C. A Mouse anti-Human Eotaxin 3 monoclonal (1 µg/ml) was used as the capture antibody.

    A = healthy patient
    B = patient with atopic dermatitis
    C = patient with bullous pemphigoid
    D = patient with allergic rhinitis

    See Abreview

References for Anti-Eotaxin 3 antibody (Biotin) (ab83505)

ab83505 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (supernatants from eosinophil granulocytes)
Specification supernatants from eosinophil granulocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type Sandwich (Detection)
Username

Abcam user community

Verified customer

Submitted Apr 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (biopsy)
Loading amount 40 µg
Specification biopsy
Gel Running Conditions Reduced Denaturing (5-16% gels)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 04 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"