Eotaxin 3 overexpression 293T lysate (whole cell) (ab94221)

Overview

  • Product nameEotaxin 3 overexpression 293T lysate (whole cell)
  • Description
    Eotaxin 3 overexpression lysate
  • General notesab94221 is a 293T cell transfected lysate in which Human Eotaxin 3 has been transiently over-expressed using a pCMV-Eotaxin 3 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Research areas
  • BackgroundFunction: Chemotactic for eosinophils and basophils. Binds to CCR3. Tissue specificity: Ubiquitously expressed at low levels in various tissues including heart and ovary. Similarity: Belongs to the intercrine beta (chemokine CC) family.

Applications

Our Abpromise guarantee covers the use of ab94221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Eotaxin 3 overexpression 293T lysate (whole cell) images

  • ab94221 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Eotaxin 3 antibody (ab67260) at 1/500 dilution

    Lane 1 : Eotaxin 3 overexpression 293T lysate (whole cell) (ab94221)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Eotaxin 3 overexpression 293T lysate (whole cell) (ab94221)

ab94221 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"