Overview

  • Product nameEotaxin Mouse ELISA Kit
    See all Eotaxin kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    1.02 pg/ml - 100 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.25 83% - 104%
    Serum 94.95 83% - 104%
    Plasma 92.54 84% - 103%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Eotaxin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Eotaxin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse Eotaxin coated on a 96-well plate. Standards and samples are pipetted into the wells and Eotaxin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Eotaxin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionIn response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    O-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • C-C motif chemokine 11
    • CCL 11
    • CCL11
    • CCL11_HUMAN
    • Chemokine (C-C motif) ligand 11
    • Chemokine C C motif ligand 11
    • Chemokine CC Motif Ligand 11
    • Chemokine ligand 11
    • Eosinophil Chemotactic P
    • Eosinophil chemotactic protein
    • Eotaxin
    • Eotaxin 1
    • MGC22554
    • SCYA 11
    • SCYA11
    • Small inducible cytokine A11
    • Small inducible cytokine subfamily A (Cys Cys) member 11
    • Small inducible cytokine subfamily A member 11
    • Small-inducible cytokine A11
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Eotaxin Mouse ELISA Kit images

  • Representative standard curve using ab100680

  • Representative standard curve using ab100680

Protocols

References for Eotaxin Mouse ELISA Kit (ab100680)

This product has been referenced in:
  • Ren X  et al. FOXM1 promotes allergen-induced goblet cell metaplasia and pulmonary inflammation. Mol Cell Biol 33:371-86 (2013). Read more (PubMed: 23149934) »
  • Ci X  et al. Different Effects of Farrerol on an OVA-Induced Allergic Asthma and LPS-induced Acute Lung Injury. PLoS One 7:e34634 (2012). Read more (PubMed: 22563373) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"