Overview

  • Product nameAnti-Epac1 antibody
    See all Epac1 primary antibodies
  • Description
    Rabbit polyclonal to Epac1
  • SpecificityAt least two isoforms of Epac1 are known to exist; this antibody will detect both isoforms. Epac1 antibody is predicted to not cross-react with Epac2.
  • Tested applicationsSuitable for: WB, ICC/IF, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 18 amino acids near the N-terminus of Human Epac1 (NP_659099).

  • Positive control
    • Rat skeletal muscle tissue lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 104 kDa.
ICC/IF Use a concentration of 20 µg/ml.
ICC Use a concentration of 2.5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • RelevanceThe activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade. There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors. EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands. Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex. The Epac 1-selective antibodies were generated against unique antigenic sequences form near N-terminus and between RasGEFN and Ras GEF domains. The antibodies to Epac 1are affinity purified over immobilized antigen based chromatography.
  • Cellular localizationEndomembrane system
  • Database links
  • Alternative names
    • bcm910 antibody
    • CAMP GEFI antibody
    • cAMP regulated guanine nucleotide exchange factor I antibody
    • CAMPGEFI antibody
    • CGEF 1 antibody
    • CGEF1 antibody
    • EPA1 antibody
    • Epac 1 antibody
    • EPAC antibody
    • EPAC1 antibody
    • Exchange factor directly activated by cAMP 1 antibody
    • Exchange protein directly activated by cAMP 1 antibody
    • MGC21410 antibody
    • RAP guanine nucleotide exchange factor antibody
    • Rap guanine nucleotide exchange factor (GEF) 3 antibody
    • RAP guanine nucleotide exchange factor 3 antibody
    • Rap1 guanine nucleotide exchange factor directly activated by cAMP antibody
    • RAPGEF3 antibody
    see all

Anti-Epac1 antibody images

  • Immunofluorescence of EPAC1 in rat brain tissue with ab124162 at 20 ug/mL.
  • Anti-Epac1 antibody (ab124162) at 1 µg/ml + Rat skeletal muscle tissue lysate at 15 µg

    Predicted band size : 104 kDa
  • Immunohistochemical analysis of rat brain tissue, staining Epac1 with ab124162 at 2.5 µg/ml.

References for Anti-Epac1 antibody (ab124162)

ab124162 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.
We can confirm that the immunogen for catalogue number ab124162 is within the first 180aa.
This antibody is guaranteed to be used in WB, other application hasn't been tested yet. if you are considering using this ...

Read More

Thank you very much for your interest in Abcam products.
I have contacted lab and asked them to release the immunogen info. I will get back to you later this evening with the info.
Many thanks for having patience!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"