Overview

  • Product name
  • Description
    Rabbit polyclonal to Epac1
  • Specificity
    ab21235 does not cross react with Epac 2.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    PRSCSYQLLLEHQRPSCI

    , corresponding to amino acids 9-26 of Epac1. Also, synthetic peptide: CRPDHSVLTLQLPVTASVR, corresponding to amino acids 522-540 of Epac1.

  • General notes
    Ab21235 is supplied in an antibody stabilisation buffer.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 99 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration. PubMed: 20663796

Target

  • Relevance
    The activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade. There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors. EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands. Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex. The Epac 1-selective antibodies were generated against unique antigenic sequences form near N-terminus and between RasGEFN and Ras GEF domains. The antibodies to Epac 1are affinity purified over immobilized antigen based chromatography.
  • Cellular localization
    Endomembrane system
  • Database links
  • Alternative names
    • bcm910 antibody
    • CAMP GEFI antibody
    • cAMP regulated guanine nucleotide exchange factor I antibody
    • CAMPGEFI antibody
    • CGEF 1 antibody
    • CGEF1 antibody
    • EPA1 antibody
    • Epac 1 antibody
    • EPAC antibody
    • EPAC1 antibody
    • Exchange factor directly activated by cAMP 1 antibody
    • Exchange protein directly activated by cAMP 1 antibody
    • MGC21410 antibody
    • RAP guanine nucleotide exchange factor antibody
    • Rap guanine nucleotide exchange factor (GEF) 3 antibody
    • RAP guanine nucleotide exchange factor 3 antibody
    • Rap1 guanine nucleotide exchange factor directly activated by cAMP antibody
    • RAPGEF3 antibody
    see all

References

This product has been referenced in:
  • Kusama K  et al. Possible roles of the cAMP-mediators EPAC and RAP1 in decidualization of rat uterus. Reproduction 147:897-906 (2014). Read more (PubMed: 24586073) »
  • Yoshie M  et al. Possible role of the exchange protein directly activated by cyclic AMP (Epac) in the cyclic AMP-dependent functional differentiation and syncytialization of human placental BeWo cells. Hum Reprod 25:2229-38 (2010). WB, IHC-P ; Human . Read more (PubMed: 20663796) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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