Our Abpromise guarantee covers the use of ab21236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 99 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 18577515|
|ICC/IF||Use a concentration of 5 µg/ml.|
ab21236 staining Epac1 in mouse pancreatic acini tissue section by Immunohistochemistry (Frozen tissue sections). Tissue underwent fixation in 4% paraformaldehyde in PBS pH 7.4 for 30 minutes, PBS washed, cryoprotected and frozen with isopenatane cooled with liquid nitrogen. The primary antibody was diluted 1/200 and incubated with sample for 2 hours at room temperature. A cy3-conjugated donkey polyclonal to rabbit IgG at 1/200 dilution was used as secondary for 1 hour. Prolong Gold with 4,6-diamino-2-phenylindole was added to mounting medium to counterstain nuclei.