Recombinant
RabMAb

Anti-Epac1 antibody [EPR1672] (ab109415)

Overview

  • Product name
    Anti-Epac1 antibody [EPR1672]
    See all Epac1 primary antibodies
  • Description
    Rabbit monoclonal [EPR1672] to Epac1
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminus of Human Epac1.

  • Positive control
    • HeLa, HepG2, Human heart and HUVEC lysates; Human kidney tissue
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR1672
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab109415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 104 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Relevance
      The activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade. There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors. EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands. Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex. The Epac 1-selective antibodies were generated against unique antigenic sequences form near N-terminus and between RasGEFN and Ras GEF domains. The antibodies to Epac 1are affinity purified over immobilized antigen based chromatography.
    • Cellular localization
      Endomembrane system
    • Database links
    • Alternative names
      • bcm910 antibody
      • CAMP GEFI antibody
      • cAMP regulated guanine nucleotide exchange factor I antibody
      • CAMPGEFI antibody
      • CGEF 1 antibody
      • CGEF1 antibody
      • EPA1 antibody
      • Epac 1 antibody
      • EPAC antibody
      • EPAC1 antibody
      • Exchange factor directly activated by cAMP 1 antibody
      • Exchange protein directly activated by cAMP 1 antibody
      • MGC21410 antibody
      • RAP guanine nucleotide exchange factor antibody
      • Rap guanine nucleotide exchange factor (GEF) 3 antibody
      • RAP guanine nucleotide exchange factor 3 antibody
      • Rap1 guanine nucleotide exchange factor directly activated by cAMP antibody
      • RAPGEF3 antibody
      see all

    Anti-Epac1 antibody [EPR1672] images

    • All lanes : Anti-Epac1 antibody [EPR1672] (ab109415) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Human heart lysate
      Lane 4 : HUVEC cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 104 kDa
    • Immunohistochemical analysis of Epac1 in paraffin-embedded Human kidney tissue using ab109415 at 1/50 dilution.

    References for Anti-Epac1 antibody [EPR1672] (ab109415)

    ab109415 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Rat Tissue lysate - whole (Cerebral Artery)
    Total protein in input
    200 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    Cerebral Artery
    Username

    Mr. Edward Humphries

    Verified customer

    Submitted May 23 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HUVEC)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    35 µg
    Specification
    HUVEC
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 21 2016

    Thank you for your inquiry.

    Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is a proprietary information. This antibody is fully guaranteed for human, mouse and rat, species. In case you ar...

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    Thank you for contacting us.
    We can confirm that the immunogen for catalogue number ab124162 is within the first 180aa.
    This antibody is guaranteed to be used in WB, other application hasn't been tested yet. if you are considering using this ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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