Overview

  • Product name
    Anti-EPAC1 delta DEP antibody
  • Description
    Rabbit polyclonal to EPAC1 delta DEP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human EPAC1 delta DEP aa 1-923.
    Database link: O95398

  • Positive control
    • HeLa, HepG2 cell line and Human heart extracts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 104 kDa.

Target

  • Function
    Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.
  • Tissue specificity
    Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney.
  • Sequence similarities
    Contains 1 cyclic nucleotide-binding domain.
    Contains 1 DEP domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 Ras-GEF domain.
  • Domain
    The DEP domain is involved in membrane localization independent from regulation by cAMP.
  • Cellular localization
    Endomembrane system.
  • Information by UniProt
  • Database links
  • Alternative names
    • bcm910 antibody
    • cAMP GEFI antibody
    • cAMP regulated guanine nucleotide exchange factor I antibody
    • cAMP-GEFI antibody
    • cAMP-regulated guanine nucleotide exchange factor I antibody
    • CGEF1 antibody
    • Epac 1 antibody
    • EPAC antibody
    • EPAC1 antibody
    • Exchange factor directly activated by cAMP 1 antibody
    • Exchange protein directly activated by cAMP 1 antibody
    • Rap guanine nucleotide exchange factor 3 antibody
    • Rap1 guanine nucleotide exchange factor directly activated by cAMP antibody
    • Rap1 guanine-nucleotide-exchange factor directly activated by cAMP antibody
    • Rapgef3 antibody
    • RPGF3_HUMAN antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab180737. Blue DAPI for nuclear staining.
  • All lanes : Anti-EPAC1 delta DEP antibody (ab180737) at 1/500 dilution

    Lane 1 : HeLa cell line extracts
    Lane 2 : HepG2 cell line extracts
    Lane 3 : Human heart extracts

    Predicted band size: 104 kDa

References

ab180737 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180737.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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