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Anti-EPAC1 delta DEP antibody
Rabbit polyclonal to EPAC1 delta DEP
Mouse, Rat, Human
Recombinant full length protein corresponding to Human EPAC1 delta DEP aa 1-923.
Database link: O95398
HeLa, HepG2 cell line and Human heart extracts.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/50 - 1/200.
1/500 - 1/2000. Predicted molecular weight: 104 kDa.
Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.
Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney.
Contains 1 cyclic nucleotide-binding domain.
Contains 1 DEP domain. Contains 1 N-terminal Ras-GEF domain. Contains 1 Ras-GEF domain.
The DEP domain is involved in membrane localization independent from regulation by cAMP.
Information by UniProt
cAMP GEFI antibody
cAMP regulated guanine nucleotide exchange factor I antibody
Immunocytochemistry/ Immunofluorescence - Anti-EPAC1 delta DEP antibody (ab180737)
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab180737. Blue DAPI for nuclear staining.
Western blot - Anti-EPAC1 delta DEP antibody (ab180737)
All lanes : Anti-EPAC1 delta DEP antibody (ab180737) at 1/500 dilution Lane 1 : HeLa cell line extracts Lane 2 : HepG2 cell line extracts Lane 3 : Human heart extracts Predicted band size : 104 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"