Anti-Epac2 antibody (ab124189)
Key features and details
- Rabbit polyclonal to Epac2
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Epac2 antibody
See all Epac2 primary antibodies -
Description
Rabbit polyclonal to Epac2 -
Host species
Rabbit -
Specificity
At least two isoforms of Epac2 are known to exist; this antibody will detect only the larger isoform. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to 15 amino acids near the N-terminus of Human Epac2 (NP_008954).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 116 kDa.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 116 kDa. |
Target
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Function
Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2. -
Tissue specificity
Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels. -
Sequence similarities
Contains 2 cyclic nucleotide-binding domains.
Contains 1 DEP domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 Ras-GEF domain. -
Domain
The N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2.
The DEP domain is involved in membrane localization independent from regulation by cAMP. -
Cellular localization
Cytoplasm. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 252857 Rat
- SwissProt: Q9Z1C7 Rat
- Unigene: 42890 Rat
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Alternative names
- CAMP GEFII antibody
- cAMP regulated guanine nucleotide exchange factor II antibody
- cAMP-GEFII antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab124189 has been referenced in 2 publications.
- Trogden KP et al. Regulation of Glucose-Dependent Golgi-Derived Microtubules by cAMP/EPAC2 Promotes Secretory Vesicle Biogenesis in Pancreatic ß Cells. Curr Biol 29:2339-2350.e5 (2019). PubMed: 31303487
- Wang SY et al. Sex Steroids Influence Brain-Derived Neurotropic Factor Secretion From Human Airway Smooth Muscle Cells. J Cell Physiol 231:1586-92 (2016). PubMed: 26566264