Overview

  • Product name
  • Description
    Rabbit polyclonal to Epac2
  • Specificity
    At least two isoforms of Epac2 are known to exist; this antibody will detect only the larger isoform.
  • Tested applications
    Suitable for: ICC, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 15 amino acids near the N-terminus of Human Epac2 (NP_008954).

  • Positive control
    • Rat liver tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab124189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 2.5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 116 kDa.Can be blocked with Epac2 peptide (ab213603).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.
  • Tissue specificity
    Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.
  • Sequence similarities
    Contains 2 cyclic nucleotide-binding domains.
    Contains 1 DEP domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 Ras-GEF domain.
  • Domain
    The N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2.
    The DEP domain is involved in membrane localization independent from regulation by cAMP.
  • Cellular localization
    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAMP GEFII antibody
    • cAMP regulated guanine nucleotide exchange factor II antibody
    • cAMP-GEFII antibody
    • cAMP-regulated guanine nucleotide exchange factor II antibody
    • CAMPGEFII antibody
    • CGEF 2 antibody
    • CGEF2 antibody
    • Epac 2 antibody
    • EPAC2 antibody
    • Exchange factor directly activated by cAMP 2 antibody
    • Exchange protein directly activated by cAMP 2 antibody
    • Nbla00496 antibody
    • Putative protein product of Nbla00496 antibody
    • Rap guanine nucleotide exchange factor (GEF) 4 antibody
    • Rap guanine nucleotide exchange factor 4 antibody
    • RAPGEF 4 antibody
    • Rapgef4 antibody
    • RPGF4_HUMAN antibody
    see all

Images

  • Immunofluorescence of Epac2 in mouse liver tissue with ab124189 at 20 ug/mL.
  • All lanes : Anti-Epac2 antibody (ab124189) at 1 µg/ml

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Rat liver tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 116 kDa
  • Immunohistochemistry of EPAC2 in mouse liver tissue with ab124189 at 2.5 ug/mL.

References

ab124189 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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