Our Abpromise guarantee covers the use of ab21238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 112 kDa.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21799930|
ab21238 staining Epac2 in the bronchial surface epithelial 16HBE14o- cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were rinsed twice with phosphate-buffered saline (PBS) and then fixed with 4% paraformaldehyde for 30 minutes at 4°C, permeabilized with 0.5% Triton X-100 in PBS for 15 minutes and blocked with 10% fetal calf serum (FCS) in PBS for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 10% FCS) overnight at 4°C. An Alex Fluor®568-conjugated Goat anti-rabbit IgG was used as the secondary antibody. The corresponding image taken in brightfield is shown on the right.
This image is courtesy of an Abreview submitted by Dr maria sabbatini