- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-Epac2 antibody
See all Epac2 primary antibodies -
DescriptionRabbit polyclonal to Epac2
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Host speciesRabbit
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SpecificityThis antibody does not cross react with other Epac family proteins.
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Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Human
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Immunogen
Synthetic peptide:
CPREQFDSLTPLPEQEGPT
, corresponding to amino acids 743-762 of Epac2
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThe affinity purified antibodies are isolated on immobilized antigen affinity column and supplied as stabilized product.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab21238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500. Predicted molecular weight: 112 kDa. | |
| ELISA | 1/10000. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 21799930 |
Target
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FunctionGuanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.
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Tissue specificityPredominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.
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Sequence similaritiesContains 2 cyclic nucleotide-binding domains.
Contains 1 DEP domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 Ras-GEF domain. -
DomainThe N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2.
The DEP domain is involved in membrane localization independent from regulation by cAMP. -
Cellular localizationCytoplasm. Membrane.
- Information by UniProt
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Database links
- Entrez Gene: 11069 Human
- Entrez Gene: 56508 Mouse
- Entrez Gene: 252857 Rat
- Omim: 606058 Human
- SwissProt: Q8WZA2 Human
- SwissProt: Q9EQZ6 Mouse
- SwissProt: Q9Z1C7 Rat
- Unigene: 470646 Human
see all -
Alternative names
- CAMP GEFII antibody
- cAMP regulated guanine nucleotide exchange factor II antibody
- cAMP-GEFII antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Epac2 antibody (ab21238)Image from Lau WK et al., PLoS One. 2011;6(7):e22363. Fig 7.; doi: 10.1371/journal.pone.0022363.ab21238 staining Epac2 in the bronchial surface epithelial 16HBE14o- cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were rinsed twice with phosphate-buffered saline (PBS) and then fixed with 4% paraformaldehyde for 30 minutes at 4°C, permeabilized with 0.5% Triton X-100 in PBS for 15 minutes and blocked with 10% fetal calf serum (FCS) in PBS for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 10% FCS) overnight at 4°C. An Alex Fluor®568-conjugated Goat anti-rabbit IgG was used as the secondary antibody. The corresponding image taken in brightfield is shown on the right.
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Western blot - Anti-Epac2 antibody (ab21238)This image is courtesy of an Abreview submitted by Dr maria sabbatiniAnti-Epac2 antibody (ab21238) at 1/1000 dilution + Whole cell lysates prepared from rabbit brain (positive control) and kidney, pancreas and pancretic acini (negative controls) at 20 µg
Secondary
HRP-conjugated goat anti-rabbit IgG
Performed under reducing conditions.
Predicted band size: 112 kDa
Exposure time: 3 minutes
References
This product has been referenced in:
- Mostafavi H et al. Selective ß2 adrenergic agonist increases Cx43 and miR-451 expression via cAMP-Epac. Mol Med Rep 9:2405-10 (2014). Human . Read more (PubMed: 24714982) »
- Guibinga GH et al. Deficiency of the purine metabolic gene HPRT dysregulates microRNA-17 family cluster and guanine-based cellular functions: a role for EPAC in Lesch-Nyhan syndrome. Hum Mol Genet 22:4502-15 (2013). Read more (PubMed: 23804752) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"