Customer reviews and FAQs

Overview

  • Product nameAnti-Epac2 antibody
    See all Epac2 primary antibodies
  • Description
    Rabbit polyclonal to Epac2
  • SpecificityThis antibody does not cross react with other Epac family proteins.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide:

    CPREQFDSLTPLPEQEGPT

    , corresponding to amino acids 743-762 of Epac2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab21238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 112 kDa.
ELISA 1/10000.
ICC/IF Use at an assay dependent concentration. PubMed: 21799930

Target

  • FunctionGuanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.
  • Tissue specificityPredominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.
  • Sequence similaritiesContains 2 cyclic nucleotide-binding domains.
    Contains 1 DEP domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 Ras-GEF domain.
  • DomainThe N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2.
    The DEP domain is involved in membrane localization independent from regulation by cAMP.
  • Cellular localizationCytoplasm. Membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • CAMP GEFII antibody
    • cAMP regulated guanine nucleotide exchange factor II antibody
    • cAMP-GEFII antibody
    • cAMP-regulated guanine nucleotide exchange factor II antibody
    • CAMPGEFII antibody
    • CGEF 2 antibody
    • CGEF2 antibody
    • Epac 2 antibody
    • EPAC2 antibody
    • Exchange factor directly activated by cAMP 2 antibody
    • Exchange protein directly activated by cAMP 2 antibody
    • Nbla00496 antibody
    • Putative protein product of Nbla00496 antibody
    • Rap guanine nucleotide exchange factor (GEF) 4 antibody
    • Rap guanine nucleotide exchange factor 4 antibody
    • RAPGEF 4 antibody
    • Rapgef4 antibody
    • RPGF4_HUMAN antibody
    see all

Anti-Epac2 antibody images

  • Immunocytochemistry/ Immunofluorescence - Anti-Epac2 antibody (ab21238)
    Immunocytochemistry/ Immunofluorescence - Anti-Epac2 antibody (ab21238)Image from Lau WK et al., PLoS One. 2011;6(7):e22363. Fig 7.; doi: 10.1371/journal.pone.0022363.

    ab21238 staining Epac2 in the bronchial surface epithelial 16HBE14o- cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were rinsed twice with phosphate-buffered saline (PBS) and then fixed with 4% paraformaldehyde for 30 minutes at 4°C, permeabilized with 0.5% Triton X-100 in PBS for 15 minutes and blocked with 10% fetal calf serum (FCS) in PBS for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 10% FCS) overnight at 4°C. An Alex Fluor®568-conjugated Goat anti-rabbit IgG was used as the secondary antibody. The corresponding image taken in brightfield is shown on the right.

  • Western blot - Anti-Epac2 antibody (ab21238)
    Western blot - Anti-Epac2 antibody (ab21238)This image is courtesy of an Abreview submitted by Dr maria sabbatini
    Anti-Epac2 antibody (ab21238) at 1/1000 dilution + Whole cell lysates prepared from rabbit brain (positive control) and kidney, pancreas and pancretic acini (negative controls) at 20 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG

    Performed under reducing conditions.

    Predicted band size : 112 kDa


    Exposure time : 3 minutes

    This image is courtesy of an Abreview submitted by Dr maria sabbatini

    See Abreview

References for Anti-Epac2 antibody (ab21238)

This product has been referenced in:
  • Mostafavi H  et al. Selective ß2 adrenergic agonist increases Cx43 and miR-451 expression via cAMP-Epac. Mol Med Rep 9:2405-10 (2014). Human . Read more (PubMed: 24714982) »
  • Guibinga GH  et al. Deficiency of the purine metabolic gene HPRT dysregulates microRNA-17 family cluster and guanine-based cellular functions: a role for EPAC in Lesch-Nyhan syndrome. Hum Mol Genet 22:4502-15 (2013). Read more (PubMed: 23804752) »

See all 5 Publications for this product

Publishing research using ab21238? Please let us know so that we can cite the reference in this datasheet.

Product Wall

Submit an abreview for ab21238 Submit a question for ab21238

3 Abreviews or Q&A

10
Votes: Highest First

Hello,
Thank you for your prompt reply, and here is the info you asked for:
1- I loaded 30ug of protein.
2- My blocking agent was 5% milk diluted in TBST; this was also what I used to dilute my primary antibody.
3- Actually, I have ...

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Thank you for providing that information.

Both the Abreview and the reference that are on the webpage for ab21238 use 5% BSA as a blocking agent and I am wondering if using that rather than 5% milk will help get a signal.

I would ad...

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I have tried using your Epac2 antibody at a 1:500 dilution for western blots, but have not been able to get any bands. In the attached picture, I ran mouse thyroid tissue lysates as well as a mouse brain lysate for a positive control. I do not expect t...

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Thank you for contacting Abcam.

I am sorry that you are having problems with ab21238 in western blot. To be able to help you more with this problem, I was wondering if you could give me a little more information:

1- How much sample ...

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Western blot abreview for Anti-Epac2 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rabbit Cell lysate - whole cell (pancreatic acini)
Loading amount 20 µg
Specification pancreatic acini
Gel Running Conditions Reduced Denaturing (7.5)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
Username

Dr. maria sabbatini

Verified customer

Submitted Sep 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"