Recombinant Anti-Epac2 antibody [EPR15246] (ab193665)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15246] to Epac2
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Epac2 antibody [EPR15246]
See all Epac2 primary antibodies -
Description
Rabbit monoclonal [EPR15246] to Epac2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Rat and Mouse brain lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15246 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa). |
Target
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Function
Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2. -
Tissue specificity
Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels. -
Sequence similarities
Contains 2 cyclic nucleotide-binding domains.
Contains 1 DEP domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 Ras-GEF domain. -
Domain
The N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2.
The DEP domain is involved in membrane localization independent from regulation by cAMP. -
Cellular localization
Cytoplasm. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 11069 Human
- Entrez Gene: 56508 Mouse
- Entrez Gene: 252857 Rat
- Omim: 606058 Human
- SwissProt: Q8WZA2 Human
- SwissProt: Q9EQZ6 Mouse
- SwissProt: Q9Z1C7 Rat
- Unigene: 470646 Human
see all -
Alternative names
- CAMP GEFII antibody
- cAMP regulated guanine nucleotide exchange factor II antibody
- cAMP-GEFII antibody
see all
Images
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All lanes : Anti-Epac2 antibody [EPR15246] (ab193665) at 1/5000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 116 kDa
Observed band size: 116 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab193665 has been referenced in 4 publications.
- Jang YN et al. RAPGEF2 mediates oligomeric Aβ-induced synaptic loss and cognitive dysfunction in the 3xTg-AD mouse model of Alzheimer's disease. Neuropathol Appl Neurobiol 47:625-639 (2021). PubMed: 33345400
- Zhong Q et al. FCPR16, a novel phosphodiesterase 4 inhibitor, produces an antidepressant-like effect in mice exposed to chronic unpredictable mild stress. Prog Neuropsychopharmacol Biol Psychiatry 90:62-75 (2019). PubMed: 30391306
- Jiang M et al. Overexpression of EPAC2 reduces the invasion of glioma cells via MMP-2. Oncol Lett 17:5080-5086 (2019). PubMed: 31186720
- Liu L et al. Epac1 agonist decreased inflammatory proteins in retinal endothelial cells, and loss of Epac1 increased inflammatory proteins in the retinal vasculature of mice. Mol Vis 23:1-7 (2017). PubMed: 28210097