Overview

  • Product name
    Anti-Epac2 antibody [EPR15246]
    See all Epac2 primary antibodies
  • Description
    Rabbit monoclonal [EPR15246] to Epac2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Epac2 aa 900 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8WZA2

  • Positive control
    • Rat and Mouse brain lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab193665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).

Target

  • Function
    Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.
  • Tissue specificity
    Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.
  • Sequence similarities
    Contains 2 cyclic nucleotide-binding domains.
    Contains 1 DEP domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 Ras-GEF domain.
  • Domain
    The N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2.
    The DEP domain is involved in membrane localization independent from regulation by cAMP.
  • Cellular localization
    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAMP GEFII antibody
    • cAMP regulated guanine nucleotide exchange factor II antibody
    • cAMP-GEFII antibody
    • cAMP-regulated guanine nucleotide exchange factor II antibody
    • CAMPGEFII antibody
    • CGEF 2 antibody
    • CGEF2 antibody
    • Epac 2 antibody
    • EPAC2 antibody
    • Exchange factor directly activated by cAMP 2 antibody
    • Exchange protein directly activated by cAMP 2 antibody
    • Nbla00496 antibody
    • Putative protein product of Nbla00496 antibody
    • Rap guanine nucleotide exchange factor (GEF) 4 antibody
    • Rap guanine nucleotide exchange factor 4 antibody
    • RAPGEF 4 antibody
    • Rapgef4 antibody
    • RPGF4_HUMAN antibody
    see all

Images

  • All lanes : Anti-Epac2 antibody [EPR15246] (ab193665) at 1/5000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Mouse brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 116 kDa
    Observed band size : 116 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:
  • Liu L  et al. Epac1 agonist decreased inflammatory proteins in retinal endothelial cells, and loss of Epac1 increased inflammatory proteins in the retinal vasculature of mice. Mol Vis 23:1-7 (2017). Read more (PubMed: 28210097) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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