Anti-EPB41 antibody (ab54597)

Overview

  • Product nameAnti-EPB41 antibody
    See all EPB41 primary antibodies
  • Description
    Mouse monoclonal to EPB41
  • Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment, corresponding to amino acids 116-226 of Human EPB41

Properties

Applications

Our Abpromise guarantee covers the use of ab54597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 µg/ml.
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionProtein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes.
  • Involvement in diseaseDefects in EPB41 are the cause of elliptocytosis type 1 (EL1) [MIM:611804]. EL1 is a Rhesus-linked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant, hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
    Defects in EPB41 are a cause of hereditary pyropoikilocytosis (HPP) [MIM:266140]. HPP is an autosomal recessive hematologic disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells.
  • Sequence similaritiesContains 1 FERM domain.
  • Post-translational
    modifications
    Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions.
    Phosphorylation on Tyr-660 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex.
    O-glycosylated; contains N-acetylglucosamine side chains in the C-terminal domain.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4.1R antibody
    • 41_HUMAN antibody
    • Band 4.1 antibody
    • E41P antibody
    • EL 1 antibody
    • EL1 antibody
    • EL1 gene antibody
    • Elliptocytosis 1 antibody
    • Elliptocytosis 1 RH linked antibody
    • EPB 4.1 antibody
    • EPB 41 antibody
    • EPB4.1 antibody
    • Epb41 antibody
    • Erythrocyte membrane protein band 4.1 (elliptocytosis 1 RH linked) antibody
    • Erythrocyte membrane protein band 4.1 antibody
    • Erythrocyte surface protein band 4.1 antibody
    • HE antibody
    • P4.1 antibody
    • Protein 4.1 antibody
    • Protein 4.1, red blood cell type antibody
    see all

Anti-EPB41 antibody images

  • EPB41 antibody (ab54597) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
  • Western blot against tagged recombinant protein immunogen using ab54597 EPB41 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

  • ab54597 at 10 µg/ml staining EPB41 in human Hella cells by Immunocytochemistry / Immunofluorescence.

  • Overlay histogram showing K562 cells stained with ab54597 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54597, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-EPB41 antibody (ab54597)

ab54597 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email.
I have added a FOCR of ab54597 in order number 1077804.
Please make sure your customers knows the 6 month guarantee period.
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Thank you for your email.
Could you provide the order number?
We have order number 929133 in system which was ordered in August 2011, this means the antibody is out of Abcam guarantee.
Looking forward to hearing from you soon.

Thank you very much for confirming the details.
It seems customer have received a faulty vial, so we are happy to offer this customer a FOCR vial from different lot. Could you confirm, if the customer would like to go ahead with this offer.
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Thank you very much for contacting us. I am sorry to hear that you are having problems using this antibody.
I have checked the protocol which seems fine however I have following questions to understand the [problem.
- Could you provide the re...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (Human erythrocyte)
Loading amount 10 µg
Specification Human erythrocyte
Gel Running Conditions Reduced Non-Denaturing (Native) (gel 10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"