Overview

  • Product nameAnti-EPB42 antibody
    See all EPB42 primary antibodies
  • Description
    Mouse monoclonal to EPB42
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: KMPEKAEQYQ PLTASVSLQN SLDAPMEDCV ISILGRGLIH RERSYRFRSV WPENTMCAKF QFTPTHVGLQ RLTVEVDCNM FQNLTNYKSV TVVAPELSA, corresponding to amino acids 623-722 of Human EPB42 with a 26kDa enzyme tag (NP_000110).

  • Positive control
    • EPB42 transfected 293T lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).

Target

  • FunctionProbably plays an important role in the regulation of erythrocyte shape and mechanical properties.
  • Involvement in diseaseDefects in EPB42 are the cause of spherocytosis type 5 (SPH5) [MIM:612690]; also known as hereditary spherocytosis type 5 (HS5). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Absence of band 4.2 associated with spur or target erythrocytes has also been reported.
  • Sequence similaritiesBelongs to the transglutaminase superfamily. Transglutaminase family.
  • Post-translational
    modifications
    Both cAMP-dependent kinase (CAPK) and another kinase present in the red-blood cells seem to be able to phosphorylate EPB42.
  • Cellular localizationCell membrane. Cytoplasm > cytoskeleton. Cytoplasmic surface of erythrocyte membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • EPB42 antibody
    • EPB42_HUMAN antibody
    • Erythrocyte membrane protein band 4.2 antibody
    • Erythrocyte protein 4.2 antibody
    • Erythrocyte surface protein band 4.2 antibody
    • MGC116735 antibody
    • MGC116737 antibody
    • P4.2 antibody
    • PA antibody
    • SPH5 antibody
    see all

Anti-EPB42 antibody images

  • Anti-EPB42 antibody (ab76910) at 1/500 dilution + Recombinant fragment of human EPB42 at 0.1 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP

    Predicted band size : 70 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-EPB42 antibody (ab76910) at 1/500 dilution

    Lane 1 : EPB42 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 70 kDa
    Observed band size : 70 kDa

References for Anti-EPB42 antibody (ab76910)

ab76910 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"