The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Antibody detection limit dilution 1/32000.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a double band of approximately 38-40 kDa (this band was successfully blocked by incubation with the immunising peptide) and the expected band of 113 kDa (predicted molecular weight: 113 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityWidely expressed.
Sequence similaritiesContains 1 FERM domain.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.