Overview

  • Product name
  • Description
    Goat polyclonal to EPB4IL2
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide:

    RREVRSPTKAPH

    (Human) from the internal region of the protein sequence according to NP_001422.1.

  • Positive control
    • Human Brain (Cerebellum) lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a double band of approximately 38-40 kDa (this band was successfully blocked by incubation with the immunising peptide) and the expected band of 113 kDa (predicted molecular weight: 113 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Contains 1 FERM domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4.1 G antibody
      • 4.1G antibody
      • AW555191 antibody
      • Band 4.1 like protein 2 antibody
      • Band 4.1-like protein 2 antibody
      • D10Ertd398e antibody
      • DKFZp781D1972 antibody
      • DKFZp781H1755 antibody
      • E41L2_HUMAN antibody
      • Epb41l2 antibody
      • Erythrocyte membrane protein band 4.1 like 2 antibody
      • Erythrocyte membrane protein band 4.1 like protein 2 antibody
      • Generally expressed protein 4.1 antibody
      • NBL2 antibody
      • OTTHUMP00000017197 antibody
      • OTTHUMP00000040262 antibody
      see all

    Anti-EPB4IL2 antibody images

    • All lanes : Anti-EPB4IL2 antibody (ab77263) at 0.1 µg/ml

      Lane 1 : Human cerebellum lysate in RIPA buffer
      Lane 2 : Human cerebellum lysate in RIPA buffer with immunising peptide

      Lysates/proteins at 35 µg/ml per lane.

      Developed using the ECL technique

      Predicted band size : 113 kDa
      Observed band size : 113 kDa
      Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-EPB4IL2 antibody (ab77263)

    ab77263 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab77263.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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