Human breast cancer MCF-7 cells.
Our Abpromise guarantee covers the use of ab85987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 35 kDa.|
|IP||Use a concentration of 1 - 4 µg/ml. per 100-500ug of protein in 1ml lysate.|
|IHC-P||Use a concentration of 1 - 10 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
|ICC||Use a concentration of 1 - 10 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|