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LoVo cell line preparation (Human).
Our Abpromise guarantee covers the use of ab20160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. Used at a dilution of 1/250 for 1 hr - see Abreview for further information.|
|Flow Cyt||Use 1µg for 106 cells.
(methanol fixed cells)
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab20160 at a 1/250 dilution staining human non small cell lung cancer cells (H358 cells) by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using an Alexa-Fluor® 546 conjugated goat anti mouse antibody.
This image is courtesy of an Abreview submitted by an anonymous researcher on 30 January 2006.