BALB/C mice were immunized with human breast carcinoma cell line, MCF-7.
Our Abpromise guarantee covers the use of ab7504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
Immunocytochemistry/ Immunofluorescence analysis of HepG2 cells labeling EpCAM with ab7504 at 1/200 dilution. Cells were fixed in methanol. Staining with ab7504 at 1/200 was carried out for 16 hours at 22°C in PBS buffer. ab150117, a Goat Anti-mouse IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody was used at 1/200 dilution. DAPI was used to counterstain.