Recombinant
RabMAb

Anti-EpCAM antibody [E144] (ab32392)

Overview

  • Product name
    Anti-EpCAM antibody [E144]
    See all EpCAM primary antibodies
  • Description
    Rabbit monoclonal [E144] to EpCAM
  • Specificity
    This antibody is specific for human EpCAM.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC,ICC/IF,IHC-Fr,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminus of Human EpCAM.

  • Positive control
    • A431 cell lysate and stomach carcinoma.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab32392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
  • Application notes
    Is unsuitable for ICC,ICC/IF,IHC-Fr,IHC-P or IP.
  • Target

    • Function
      May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
    • Tissue specificity
      Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
    • Involvement in disease
      Defects in EPCAM are the cause of diarrhea type 5 (DIAR5) [MIM:613217]. It is an intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum.
      Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM.
    • Sequence similarities
      Belongs to the EPCAM family.
      Contains 1 thyroglobulin type-1 domain.
    • Post-translational
      modifications
      Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
    • Cellular localization
      Lateral cell membrane. Cell junction > tight junction. Co-localizes with CLDN7 at the lateral cell membrane and tight junction.
    • Information by UniProt
    • Database links
    • Alternative names
      • 17 1A antibody
      • 323/A3 antibody
      • Adenocarcinoma associated antigen antibody
      • Adenocarcinoma-associated antigen antibody
      • Antigen identified by monoclonal antibody AUA1 antibody
      • AUA1 antibody
      • CD326 antibody
      • CD326 antigen antibody
      • Cell surface glycoprotein Trop 1 antibody
      • Cell surface glycoprotein Trop 2 antibody
      • Cell surface glycoprotein Trop-1 antibody
      • CO 17A antibody
      • CO17 1A antibody
      • CO17A antibody
      • DIAR5 antibody
      • EGP 2 antibody
      • EGP antibody
      • EGP2 antibody
      • EGP314 antibody
      • EGP40 antibody
      • Ep CAM antibody
      • Ep-CAM antibody
      • EPCAM antibody
      • EPCAM_HUMAN antibody
      • EpCAM1 antibody
      • Epithelial cell adhesion molecule antibody
      • Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) antibody
      • Epithelial cell surface antigen antibody
      • Epithelial cellular adhesion molecule antibody
      • Epithelial glycoprotein 1 antibody
      • Epithelial glycoprotein 314 antibody
      • Epithelial glycoprotein antibody
      • ESA antibody
      • GA733 1 antibody
      • GA733 2 antibody
      • GA733-2 antibody
      • gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein antibody
      • gp4 antibody
      • hEGP 2 antibody
      • hEGP314 antibody
      • HNPCC8 antibody
      • Human epithelial glycoprotein 2 antibody
      • KS 1/4 antigen antibody
      • KS1/4 antibody
      • KSA antibody
      • Ly74 antibody
      • Lymphocyte antigen 74 antibody
      • M1S 1 antibody
      • M1S2 antibody
      • M4S1 antibody
      • Major gastrointestinal tumor associated protein GA733 2 antibody
      • Major gastrointestinal tumor-associated protein GA733-2 antibody
      • mEGP314 antibody
      • Membrane component chromosome 4 surface marker (35kD glycoprotein) antibody
      • Membrane component, chromosome 4, surface marker 1 antibody
      • Membrane component, chromosome 4, surface marker antibody
      • MIC18 antibody
      • MK 1 antibody
      • Protein 289A antibody
      • TACD1 antibody
      • TACSTD1 antibody
      • TROP1 antibody
      • Tumor associated calcium signal transducer 1 antibody
      • Tumor associated calcium signal transducer 2 precursor antibody
      • Tumor-associated calcium signal transducer 1 antibody
      see all

    Anti-EpCAM antibody [E144] images

    • Anti-EpCAM antibody [E144] (ab32392) at 1/2500 dilution + A431 cell lysate

      Predicted band size : 39 kDa

    Protocols

    References for Anti-EpCAM antibody [E144] (ab32392)

    This product has been referenced in:
    • Sukhbaatar N  et al. Two different, mutually exclusively distributed, TP53 mutations in ovarian and peritoneal tumor tissues of a serous ovarian cancer patient: indicative for tumor origin? Cold Spring Harb Mol Case Stud 3:N/A (2017). ICC/IF ; Human . Read more (PubMed: 28679689) »
    • Thakkar A  et al. Vitamin D and androgen receptor-targeted therapy for triple-negative breast cancer. Breast Cancer Res Treat 157:77-90 (2016). Read more (PubMed: 27120467) »

    See all 29 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (small intestine organoids)
    Specification
    small intestine organoids
    Username

    Abcam user community

    Verified customer

    Submitted Mar 24 2017

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Adenocarcinoma cells)
    Permeabilization
    Yes - 0.1% TX-100
    Specification
    Adenocarcinoma cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 09 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Aug 25 2016

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (stomach)
    Permeabilization
    No
    Specification
    stomach
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    Submitted Aug 22 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    skin tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Aug 15 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate buffer pH6
    Permeabilization
    No
    Specification
    tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 11 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Ovarian Tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6
    Permeabilization
    No
    Specification
    Ovarian Tumor
    Blocking step
    Peroxidase Blocking Solution + UV block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    58°C - 1h
    Username

    Abcam user community

    Verified customer

    Submitted Jun 02 2016

    Application
    ELISA
    Sample
    Human Cell (Cancer cell line)
    Specification
    Cancer cell line
    Blocking step
    PBS as blocking agent for 30 minute(s) · Concentration: 15µg/mL · Temperature: 37°C
    Type
    Direct
    Username

    Abcam user community

    Verified customer

    Submitted Sep 18 2015

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Colon cancer cell lines)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    Colon cancer cell lines
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 30 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Apr 04 2014

    1-10 of 42 Abreviews or Q&A

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