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Living human colon carcinoma cell line HT-29
ab46714 reacts intensely with all carcinomas and metastased derived from the intestinal tract, stomach, pancreas, liver, lung, breast, ovary, thyroid gland, kidney, bladder, prostate. Stratified squamous epithelia usually stain less intensely than adenocarcinoma. The antibody is suitable for differentiation between carcinoma and non-carcinoma. Keratinizing areas of a tumour mass usually remain unstained, as do sarcoma, lymphoma, melanoma and neurogenic tumours.
Our Abpromise guarantee covers the use of ab46714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
ICC/IF image of ab46714 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA, 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46714 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.