Tail base Human EPCR/CD201-positive RE-1 cells
Ablates APC binding to EPCR/CD201 thus blocking APC-mediated signalling.
Our Abpromise guarantee covers the use of ab81712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Inhibition Assay||Use a concentration of 10 - 25 µg/ml. Antibody RCR-252 functions as blocking antbody. It ablates APC binding to EPCR and blocks APC-mediated signaling.|
|Flow Cyt||1/50. ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
|WB||1/50. Use under non reducing condition.|
Detection of EPCR in HUVEC cells. Red and black line represent the isotype control and ab81712 at 20 μg/ml, respectively.