Anti-Eph receptor A2 antibody [3D7] (ab118882)

Overview

  • Product nameAnti-Eph receptor A2 antibody [3D7]
    See all Eph receptor A2 primary antibodies
  • Description
    Mouse monoclonal [3D7] to Eph receptor A2
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 559-976 of Human Eph receptor A2 (NP_004422).

  • Positive control
    • WB: Mouse brain extracts IHC-P: Human testis, thyroid, colon cancer and gastric cancer tissues.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab118882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 108 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionReceptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5. Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Induces apoptosis in a p53/TP53-independent, caspase-8-dependent manner.
  • Tissue specificityExpressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary.
  • Involvement in diseaseGenetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2 (ARCC2) [MIM:613020]. A developmental punctate opacity common in the cortex and present in most lenses. The cataract is white or cerulean, increases in number with age, but rarely affects vision.
    Defects in EPHA2 are the cause of cataract posterior polar type 1 (CTPP1) [MIM:116600]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational
    modifications
    Activated by EFNA1 via tyrosine phosphorylation. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit. These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and endothelial cell migration. They also play a critical role in transducing EPHA2 signaling in vascular endothelial cells during tumor angiogenesis.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARCC2 antibody
    • AW545284 antibody
    • CTPA antibody
    • CTPP1 antibody
    • CTRCT6 antibody
    • EC 2.7.10.1 antibody
    • Eck antibody
    • Eph receptor A2 antibody
    • EPHA2 antibody
    • EPHA2_HUMAN antibody
    • Ephrin receptor antibody
    • Ephrin receptor EphA2 antibody
    • Ephrin type A receptor 2 antibody
    • Ephrin type-A receptor 2 antibody
    • Epithelial cell kinase antibody
    • Epithelial cell receptor protein tyrosine kinase antibody
    • Myk 2 antibody
    • Myk2 antibody
    • Sek 2 antibody
    • Sek2 antibody
    • Soluble EPHA2 variant 1 antibody
    • Tyrosine protein kinase receptor ECK antibody
    • Tyrosine-protein kinase receptor ECK antibody
    • Tyrosine-protein kinase receptor MPK-5 antibody
    • Tyrosine-protein kinase receptor SEK-2 antibody
    see all

Anti-Eph receptor A2 antibody [3D7] images

  • Anti-Eph receptor A2 antibody [3D7] (ab118882) at 1/500 dilution + Mouse brain extracts at 50 µg

    Secondary
    Goat anti-Mouse conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 108 kDa
  • Human testis (formalin fixed, paraffin embedded) stained with ab118882 at 5 µg/ml, followed by biotinylated anti-Mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Human thyroid tissue (formalin fixed, paraffin embedded) stained with ab118882 at 5 µg/ml, followed by biotinylated anti-Mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Paraffin embedded sections of Human colon cancer tissue were incubated with ab118882 (1/100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • Paraffin embedded sections of Human gastric cancer tissue were incubated with ab18882 (1/100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).

References for Anti-Eph receptor A2 antibody [3D7] (ab118882)

ab118882 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (undifferentiated Human ES cells on MEFs)
Permeabilization Yes - see blocking buffer
Specification undifferentiated Human ES cells on MEFs
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative Paraformaldehyde
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Submitted Apr 05 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HUES7 ES cells)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 20 µg
Specification HUES7 ES cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Submitted Feb 22 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEPG2)
Specification HEPG2
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative Paraformaldehyde
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Submitted Nov 18 2015

Application Western blot
Sample Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions Reduced Denaturing (12.5)
Loading amount 20 µg
Specification HEPG2
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Submitted Nov 13 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step 1% serum + 0.1% BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Sample Mouse Cell (mouse embyonic stem cells)
Specification mouse embyonic stem cells
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
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Submitted Apr 23 2014

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization No
Sample Mouse Cell (mouse embryonic stem cells)
Specification mouse embryonic stem cells
Gating Strategy live cells in fsc vs ssc
Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.2% BSA, 0.1% NaAz in PBS
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Submitted Mar 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"