Anti-Eph receptor A2+A3+A4 (phospho Y588 + Y596) antibody (ab62256)

Overview

  • Product name
    Anti-Eph receptor A2+A3+A4 (phospho Y588 + Y596) antibody
    See all Eph receptor A2+A3+A4 primary antibodies
  • Description
    Rabbit polyclonal to Eph receptor A2+A3+A4 (phospho Y588 + Y596)
  • Specificity
    ab62256 detects endogenous levels of Eph receptor A2+A3+A4 only when phosphorylated at tyrosine 588/596.
  • Tested applications
    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Sea urchin
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human Eph receptor A2+A3+A4 around the phosphorylation site of tyrosine 588/596 (T-YP-V-D-P).

  • Positive control
    • HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

Images

  • ab62256 at 1/500 - 1/1000 dilution staining Eph receptor A2+A3+A4 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

References

This product has been referenced in:
  • Mansour M  et al. PTP-PEST controls EphA3 activation and ephrin-induced cytoskeletal remodelling. J Cell Sci 129:277-89 (2016). WB ; Human . Read more (PubMed: 26644181) »
  • Sabet O  et al. Ubiquitination switches EphA2 vesicular traffic from a continuous safeguard to a finite signalling mode. Nat Commun 6:8047 (2015). Read more (PubMed: 26292967) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Sea Urchin Cell (Embryo, Strongylocentrotus purpuratus)
Specification
Embryo, Strongylocentrotus purpuratus
Fixative
Paraformaldehyde
Permeabilization
Yes - Superblock with 0.03% TritonX-100, 1 mM Na3VO4, 1 mM NaF
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Dr. Robert Burke

Verified customer

Submitted Mar 28 2013

Thank you for contacting us.

The lab sent me more information about the immunogen of the antibody ab62256:

The antibody is against both EPHA2 and EPHA3. This antibody recognizes EPHA2 when it is phosphorylated at Y588 and EPHA3 when i...

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Thank you for contacting us.

The immunogen for the anti-phosphorylated Eph receptor antibody, ab62256, is identical to the sequence you provided, at least around Y588. I have asked the laboratory to provide a more precise alignment (I do not...

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Thank you for contacting us. I am sorry to hear that this antibody did not work as expected. I can certainly arrange a refund for you, but would like to gather some more information about the actual problem for our records. Please let me know: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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