Anti-Eph receptor A3 antibody (ab54623)


  • Product name
    Anti-Eph receptor A3 antibody
    See all Eph receptor A3 primary antibodies
  • Description
    Mouse monoclonal to Eph receptor A3
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 202-326 of Human Eph receptor A3



Our Abpromise guarantee covers the use of ab54623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 110 kDa.
IHC-P Use a concentration of 5 µg/ml.


  • Function
    Receptor for members of the ephrin-A family. Binds to ephrin-A2, -A3, -A4 and -A5. Could play a role in lymphoid function.
  • Tissue specificity
    Widely expressed. Highest level in placenta.
  • Involvement in disease
    Defects in EPHA3 may be a cause of colorectal cancer (CRC) [MIM:114500].
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW492086 antibody
    • Cek4 antibody
    • EC antibody
    • EK4 antibody
    • End3 antibody
    • Eph receptor A3 antibody
    • EPH-like kinase 4 antibody
    • EPH-like tyrosine kinase 1 antibody
    • EPHA3 antibody
    • EPHA3_HUMAN antibody
    • Ephrin receptor EphA3 antibody
    • Ephrin type-A receptor 3 antibody
    • ETK 1 antibody
    • ETK antibody
    • ETK1 antibody
    • HEK 4 antibody
    • HEK antibody
    • HEK4 antibody
    • Human embryo kinase 1 antibody
    • Human embryo kinase antibody
    • Mek4 antibody
    • MGC109882 antibody
    • Receptor tyrosine kinase HEK antibody
    • Testicular tissue protein Li 64 antibody
    • Tyro 4 antibody
    • Tyro4 antibody
    • TYRO4 protein tyrosine kinase antibody
    • Tyrosine protein kinase receptor ETK 1 antibody
    • Tyrosine-protein kinase receptor ETK1 antibody
    • Tyrosine-protein kinase TYRO4 antibody
    see all

Anti-Eph receptor A3 antibody images

  • Predicted band size : 110 kDa
    Eph receptor A3 antibody (ab54623) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ICC/IF image of ab54623 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54623, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab54623 staining in Human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab54623, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Eph receptor A3 antibody (ab54623)

ab54623 has not yet been referenced specifically in any publications.

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Thank you for your enquiry.

I am very pleased to hear the customer would like make use of ourtesting discountoffer and test ab54623 Anti-Eph receptor A3 antibody in flow cytometry.

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Thank you for contacting us.
You are right; the immunogen is indeed present in the extracellular domain of the protein so there is no need for permeabilization. You may skip this step.
We unfortunately do not have any additional data for shar...

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